Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,596,637	A→G	S323P (TCG→CCG)	ftsY ←	signal recognition particle‑docking protein FtsY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,596,637	0	A	G	100.0%	60.3 / NA	18	S323P (TCG→CCG)	ftsY	signal recognition particle‑docking protein FtsY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/11);  total (7/11)

ATATTGTTGCGCTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCA  >  NZ_CP009273/3596558‑3596714                                                                                |                                                                              atatTGTTGCGCTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGGTTTACCctgc                                                                     <  2:423841/90‑1 (MQ=255)           gcTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGGTTTACCCTGCTGCTCAAACt                                                           >  1:343010/1‑90 (MQ=255)            cTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGGTTTACCCTGCTGCTCAAACTg                                                          <  1:453907/90‑1 (MQ=255)              gACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGGTTTACCCTGCTGCTCAAACTGAc                                                        >  1:415021/1‑90 (MQ=255)              gACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGGTTTACCCTGCTGCTCAAACTGAc                                                        >  1:430747/1‑90 (MQ=255)                        cTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGGTTTACCCTGCTGCTCAAACTGACGCGCCAGCtt                                              >  2:309075/1‑90 (MQ=255)                          gAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGGTTTACCCTGCTGCTCAAACTGACGCGCCAGCTTAc                                            >  2:465513/1‑90 (MQ=255)                          gAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGGTTTACCCTGCTGCTCAAACTGACGCGCCAGCTTAc                                            >  2:36847/1‑90 (MQ=255)                              cTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGGTTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAAt                                        <  1:36847/90‑1 (MQ=255)                               tGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGGTTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAAtc                                       >  1:325922/1‑90 (MQ=255)                                    acccGCAGCTGCCCGGAAAGTCTCCCCCGCCGCCAGCATCACCGGTTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATcgtcgt                                  <  2:325922/88‑1 (MQ=255)                                              gCACGGAAAGTATCACCCGCCGCCAGCATCACCGGTTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTAccc                        <  2:126598/90‑1 (MQ=255)                                                         aTCACCCGCCGCCAGCATCACCGGTTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGAc             <  1:86490/90‑1 (MQ=255)                                                            ccccGCCGCCAGCATCACCGGTTTACCCTGCTGATCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTCCCCACACCGTTGACGcc          <  1:435217/89‑1 (MQ=255)                                                             cccGCCGCCAGCATCACCGGTTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGccc         <  2:384223/90‑1 (MQ=255)                                                              ccgccgCCAGCATCACCGGTTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCcca        <  2:70600/90‑1 (MQ=255)                                                                   gccAGCATCACCGGTTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATc   <  2:343010/90‑1 (MQ=255)                                                                    ccAGCATCACCGGTTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCa  <  1:309075/90‑1 (MQ=255)                                                                                |                                                                              ATATTGTTGCGCTGACCCCAGACCTGAAGCTGTTCAACCGCAGCTGCACGGAAAGTATCACCCGCCGCCAGCATCACCGATTTACCCTGCTGCTCAAACTGACGCGCCAGCTTACCAATCGTCGTGGTTTTACCCACACCGTTGACGCCCACCATCA  >  NZ_CP009273/3596558‑3596714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑