| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,625,150 | T→C | R267R (AGA→AGG) | yhiJ ← | YhiJ/YhiL family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,625,150 | 0 | T | C | 92.9% | 39.7 / ‑3.3 | 14 | R267R (AGA→AGG) | yhiJ | YhiJ/YhiL family protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (5/8); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTTCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTAAGTTGCTCATTAAAGTTCTCCCTTAGCAATACGTTGAGAACTTCA > NZ_CP009273/3625067‑3625234 | tGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTCCTGTTc < 1:229068/90‑1 (MQ=255)tGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTCCTGTTc < 2:365546/90‑1 (MQ=255) ggTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTCCTGTTCa < 1:17234/90‑1 (MQ=255) ccAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTCCTGTTCAGGATAc < 2:1726/90‑1 (MQ=255) aaaCGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTCCTGTTCAGGATACTGat < 1:59371/90‑1 (MQ=255) gTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTCCTGTTCAGGATACTGattatt < 2:153189/90‑1 (MQ=255) gCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTCCTGTTCAGGATACTGATTATTGTATTaa > 1:167032/1‑90 (MQ=255) gCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTCCTGTTCAGGATACTGATTATTGTATTaa > 1:303298/1‑90 (MQ=255) tCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTCCTGTTCAGGATACTGATTATTGTATTAACAt > 2:450333/1‑90 (MQ=255) aTAAGGCTGAAGACTTTCTAAAATAATGTCTCTTCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTAAGTTGCTCATTAAAGtt < 1:31792/90‑1 (MQ=255) gAAGACTTTCTAAAATAATGTCTCTCCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTAAGTTGCTCATTAAAGTTCTCCCTTa > 1:400295/1‑90 (MQ=255) cTAAAATAATGTCTCTCCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTAAGTTGCTCATTAAAGTTCTCCCTTAGCAATACGt < 2:181651/90‑1 (MQ=255) atGTCTCTCCTGTTCAGGATACTGATTATTGTATTa > 1:286692/1‑36 (MQ=38) atGTCTCTCCTGTTCAGGATACTGATTATTGTATTa < 2:286692/36‑1 (MQ=38) tctctcCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTAAGTTGCTCATTAAAGTTCTCCCTTAGCAATACGTTGAGAACTTCa < 2:279897/90‑1 (MQ=255) tctctcCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTAAGTTGCTCATTAAAGTTCTCCCTTAGCAATACGTTGAGAACTTCa < 2:303298/90‑1 (MQ=255) | TGGTCGCCCAGAAACGTACATGGCGTCACAGCGTCTTTATTAATAACTAAATAAGGCTGAAGACTTTCTAAAATAATGTCTCTTCTGTTCAGGATACTGATTATTGTATTAACATTAGTGTTAAGTTGCTCATTAAAGTTCTCCCTTAGCAATACGTTGAGAACTTCA > NZ_CP009273/3625067‑3625234 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |