Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,660,711	T→C	D77G (GAT→GGT)	gadA ←	glutamate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,660,711	0	T	C	100.0%	91.5 / NA	26	D77G (GAT→GGT)	gadA	glutamate decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/13);  total (13/13)

CGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTATTGATCGACAAATCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAA  >  NZ_CP009273/3660625‑3660786                                                                                       |                                                                            cGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTATTGATCGACAAACCCa                                                                          >  1:511036/1‑90 (MQ=255)   gCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTATTGATCGACAAACCCATc                                                                        >  1:113270/1‑90 (MQ=255)   gCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTATTGATCGACAAACCCATc                                                                        >  1:294952/1‑90 (MQ=255)   gCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTATTGATCGACAAACCCATc                                                                        <  2:161581/90‑1 (MQ=255)               acgcaacgcaGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTATTGATCGACAAACCCATCAATTTATGGACg                                                            >  1:5676/1‑90 (MQ=255)                   aacgcaGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTATTGATCGACAAACCCATCAATTTATGGACGtttt                                                        >  1:109555/1‑90 (MQ=255)                    acgcaGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTATTGATCGACAAACCCATCAATTTATGGACGTTTtc                                                       >  1:196130/1‑90 (MQ=255)                    acgcaGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTATTGATCGACAAACCCATCAATTTATGGACGTTTtc                                                       >  2:1966/1‑90 (MQ=255)                    acgcaGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTATTGATCGACAAACCCATCAATTTATGGACGTTTtc                                                       >  1:106909/1‑90 (MQ=255)                             gATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCa                                              <  2:264145/90‑1 (MQ=255)                                 gCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTc                                          <  1:214310/90‑1 (MQ=255)                                 gCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTc                                          <  2:113270/90‑1 (MQ=255)                                   tgcggattgcggatATTCTTCTTTGTCGATCCAGTTTTTATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTCTg                                        >  2:325872/1‑90 (MQ=255)                                                     ttcttTGTCGATCCAGTTTTTATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGtt                      <  2:109589/90‑1 (MQ=255)                                                      tcttTGTCGATCCAGTTTTTATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTc                     <  2:106909/90‑1 (MQ=255)                                                        tttGTCGATCCAGTTTTTATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTg                   >  2:406181/1‑90 (MQ=255)                                                          tGTCGATCCAGTTTTTATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGAc                 <  1:84307/90‑1 (MQ=255)                                                             cGATCCAGTTTTTATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAg              >  2:173458/1‑90 (MQ=255)                                                                      ttttATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCAt     <  2:5676/90‑1 (MQ=255)                                                                       tttATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATc    <  1:325872/90‑1 (MQ=255)                                                                       tttATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATc    <  2:218717/90‑1 (MQ=255)                                                                         tATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCa                          <  2:110555/66‑1 (MQ=255)                                                                         tATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCa                          >  1:110555/1‑66 (MQ=255)                                                                         tATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATc    >  1:292182/1‑88 (MQ=255)                                                                         tATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATc    <  2:292182/88‑1 (MQ=255)                                                                         tATTGATCGACAAACCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCaa  <  2:418371/90‑1 (MQ=255)                                                                                       |                                                                            CGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTATTGATCGACAAATCCATCAATTTATGGACGTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAA  >  NZ_CP009273/3660625‑3660786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑