Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,810,692	T→A	D219E (GAT→GAA)	yicC →	YicC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,810,692	0	T	A	100.0%	49.3 / NA	14	D219E (GAT→GAA)	yicC	YicC family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (9/5);  total (9/5)

AGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGG  >  NZ_CP009273/3810605‑3810777                                                                                        |                                                                                      aGATGCTCAGGTGCAACTGGAAAACAACCGTCTTGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGAACg                                                                                     <  2:456957/90‑1 (MQ=255)     gCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGAACGCCTc                                                                                 >  1:299173/1‑90 (MQ=255)             gCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGAACGCCTCGAAGCGCa                                                                         <  1:411813/90‑1 (MQ=255)                    gAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGAACGCCTCGAAGCGCATGTCaaa                                                                  >  2:117607/1‑90 (MQ=255)                        acaccCGTCCGGCGCAGGAACTGGTTCTGCTAACACAACGAATTGAAGTTGCCGAAGAACTGGAACGCCTCGAAGCGCATGTCAAAgaga                                                              <  2:436133/90‑1 (MQ=255)                        acaacCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGAACGCCTCGAAGCGCATGTCAAAgaga                                                              >  1:114690/1‑90 (MQ=255)                               tCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGAACGCCTCGAAGCGCATGTCAAAGAGACCTacaa                                                       <  2:247066/90‑1 (MQ=255)                                     gCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGAACGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCt                                                 >  1:419817/1‑90 (MQ=255)                                        ggAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGAACGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTgaa                                              >  1:76206/1‑90 (MQ=255)                                                                  ttGACGTTGCCGAAGAACTGGAACGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGgtcgtcgtc                    <  1:275626/90‑1 (MQ=255)                                                                          gCCGAAGAACTGGAACGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGAtttt            >  1:291678/1‑90 (MQ=255)                                                                              aagaaCTGGAACGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTatga        >  2:301471/1‑90 (MQ=255)                                                                                    tGGAACGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAgg  >  1:105096/1‑90 (MQ=255)                                                                                    tGGAACGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAgg  >  2:517327/1‑90 (MQ=255)                                                                                        |                                                                                      AGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTGGATTTTATGATGCAGG  >  NZ_CP009273/3810605‑3810777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑