Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,875,083	A→G	V200A (GTG→GCG)	dnaN ←	DNA polymerase III subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,875,083	0	A	G	100.0%	31.2 / NA	10	V200A (GTG→GCG)	dnaN	DNA polymerase III subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/5);  total (5/5)

TGGGCGCGAATGTTGTTGCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCG  >  NZ_CP009273/3875005‑3875166                                                                               |                                                                                    tGGGCGCGAATGTTGTTGCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCGCGCCTTTAcgc                                                                          >  2:5468/1‑90 (MQ=255)                  gctgcCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCGCGCCTTTACGCGGTACGATCACCGAATg                                                         >  2:442711/1‑90 (MQ=255)                                  cgcAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCGCGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGa                                         >  1:229729/1‑90 (MQ=255)                                     aGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCGCGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCa                                      >  2:104841/1‑90 (MQ=255)                                        ggATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCGCGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAAtt                                   <  1:255790/90‑1 (MQ=255)                                        ggATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCGCGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAAtt                                   <  1:274667/90‑1 (MQ=255)                                                          aGCATACGCATCAGTTCAATCGCGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGcc                 <  2:246280/90‑1 (MQ=255)                                                              tACGCATCAGTTCAATCGCGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGAc             >  2:239944/1‑90 (MQ=255)                                                                         tCAATCGCGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCg  <  1:161861/90‑1 (MQ=255)                                                                         tCAATCGCGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCg  <  2:414652/90‑1 (MQ=255)                                                                               |                                                                                    TGGGCGCGAATGTTGTTGCTGCCAATCTGTACGCGCAGCGGATTGTCGCCGCCGTCGAGCATACGCATCAGTTCAATCACGCCTTTACGCGGTACGATCACCGAATGGCTTGGCAAAGATTGACCAATTGGCATTGAACAGACCGCCAGACGGTGGCCGTCG  >  NZ_CP009273/3875005‑3875166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑