Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,879,837	C→T	P468S (CCG→TCG)	yidC →	membrane protein insertase YidC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,879,837	0	C	T	100.0%	125.5 / NA	34	P468S (CCG→TCG)	yidC	membrane protein insertase YidC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/17);  total (17/17)

ATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACC  >  NZ_CP009273/3879750‑3879923                                                                                        |                                                                                       aTGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGTCg                                                                                      >  1:153974/1‑90 (MQ=255)   gCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGTCGAt                                                                                    <  1:438110/90‑1 (MQ=255)     tGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGTCGATcc                                                                                  >  2:10897/1‑90 (MQ=255)      gATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGTCGATCCt                                                                                 >  2:273545/1‑90 (MQ=255)         gggTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGTCGATCCTGAt                                                                              >  1:216984/1‑90 (MQ=255)          ggTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGTCGATCCTGATg                                                                             <  2:80598/90‑1 (MQ=255)          ggTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGTCGATCCTGATg                                                                             <  2:345130/90‑1 (MQ=255)           gTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGTCGATCCTGATgg                                                                            >  1:20210/1‑90 (MQ=255)               cGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGTCGATCCTGATGGGCGt                                                                        <  2:393586/90‑1 (MQ=255)                                               ggATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGa                                        <  2:19615/90‑1 (MQ=255)                                                gATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGAc                                       <  1:155835/90‑1 (MQ=255)                                                 aTCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGAcc                                      <  1:273545/90‑1 (MQ=255)                                                     aCGACCTGTCGGCACAGGACCCGTACTACATCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAg                                  >  1:331723/1‑90 (MQ=255)                                                     aCGACCTGTCGGCACAGGACCCGTACTACATCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAg                                  >  2:220076/1‑90 (MQ=255)                                                       gACCTGTCGGCACAGGACCCGTACTACATCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTg                                >  1:37909/1‑90 (MQ=255)                                                          cTGTCGGCACAGGACCCGTACTACATCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTgacc                             <  2:42692/90‑1 (MQ=255)                                                          cTGTCGGCACAGGACCCGTACTACATCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTgacc                             >  2:302578/1‑90 (MQ=255)                                                            gTCGGCACAGGACCCGTACTACATCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTgaccga                           >  2:263589/1‑90 (MQ=255)                                                            gTCGGCACAGGACCCGTACTACATCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTgaccga                           >  2:241794/1‑90 (MQ=255)                                                                  acaGGACCCGTACTACATCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGAt                     <  2:34943/90‑1 (MQ=255)                                                                    aGGACCCGTACTACATCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATgc                   <  2:523557/90‑1 (MQ=255)                                                                          cGTACTACATCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCaga             >  1:118103/1‑90 (MQ=255)                                                                          cGTACTACATCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCaga             >  2:107104/1‑90 (MQ=255)                                                                           gTACTACATCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCagaa            <  1:370918/90‑1 (MQ=255)                                                                                  ctCCTGTCGTTCCTGATGGTCGTAACGAAGTTCTTCATTCACAAAATGTCGCCGACCACTGTCATCGACACGATTCAGCAGAAGATCATg     <  2:18168/89‑1 (MQ=255)                                                                                  aTCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATg     <  2:190387/90‑1 (MQ=255)                                                                                  aTCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATg     >  1:190387/1‑90 (MQ=255)                                                                                  aTCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATg     <  1:173497/90‑1 (MQ=255)                                                                                  aTCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATg     >  1:58812/1‑90 (MQ=255)                                                                                  aTCCTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATg     <  1:57165/90‑1 (MQ=255)                                                                                     cTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGAt                     <  1:64959/71‑1 (MQ=255)                                                                                     cTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATg     <  2:372104/87‑1 (MQ=255)                                                                                     cTGTCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATg     >  1:372104/1‑87 (MQ=255)                                                                                     cTGTCGATCCTGATGGCCGCAACGATGTCCTTCATTCAGACGCTTTCCCCGACCACAGCGACCAACCCGATGCAGCAGAGGATCATGAcc  >  1:231150/1‑90 (MQ=255)                                                                                        |                                                                                       ATGCTGATGGGTTCCGTTGAACTGCGTCAGGCACCGTTTGCACTGTGGATCCACGACCTGTCGGCACAGGACCCGTACTACATCCTGCCGATCCTGATGGGCGTAACGATGTTCTTCATTCAGAAGATGTCGCCGACCACAGTGACCGACCCGATGCAGCAGAAGATCATGACC  >  NZ_CP009273/3879750‑3879923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑