Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,926,018	T→C	L448P (CTG→CCG)	kup →	low affinity potassium transporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,926,018	0	T	C	100.0%	67.0 / NA	20	L448P (CTG→CCG)	kup	low affinity potassium transporter Kup
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/8);  total (12/8)

TGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGAC  >  NZ_CP009273/3925931‑3926102                                                                                        |                                                                                     tGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTtgccgc                                                                                    <  2:343970/90‑1 (MQ=255) tGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTtgccgc                                                                                    <  2:333425/90‑1 (MQ=255)  gCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCcgcg                                                                                   >  1:286805/1‑90 (MQ=255)                ggTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCCGCGGCGGATGCATGAAc                                                                     <  1:226987/90‑1 (MQ=255)                      cATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTa                                                               <  2:100340/90‑1 (MQ=255)                        ttGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTAAc                                                             <  2:486152/90‑1 (MQ=255)                           aGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTAActct                                                          >  2:371251/1‑90 (MQ=255)                               tCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTAACTCTCTgg                                                      >  2:319583/1‑90 (MQ=255)                                cGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTAACTCTCTGGa                                                     <  2:67617/90‑1 (MQ=255)                                 ggTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTAACTCTCTGGaa                                                    >  2:363874/1‑90 (MQ=255)                                       gtgATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCgatg                                              >  1:406284/1‑90 (MQ=255)                                            gTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGc                                         >  1:87655/1‑90 (MQ=255)                                            gTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGc                                         >  2:175087/1‑90 (MQ=255)                                            gTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGc                                         >  1:321101/1‑90 (MQ=255)                                            gTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGc                                         <  1:266402/90‑1 (MQ=255)                                               tATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTc                                      >  2:110679/1‑90 (MQ=255)                                                       tgaCCACCTGGAAGAGCGAGCGTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGgaga                              <  2:504956/90‑1 (MQ=255)                                                             ccTGGAAGAGCGAGCGTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTAAc                                                             >  2:25477/1‑53 (MQ=255)                                                             ccTGGAAGAGCGAGCGTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTAAc                                                             <  1:25477/53‑1 (MQ=255)                                                                      gcgagcgTTTCCGCTTGCCGCGGCGGATGCATGAAGAAGGAAAATCGCTGGGGGGGATGATTGCTTCGCGAGAGAAAGCACCGCGTGTTc               >  2:240342/1‑90 (MQ=255)                                                                      gcgagcgTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTc               >  1:346863/1‑90 (MQ=255)                                                                       cgagcgTTTCCGCTTGCCGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTcg              >  2:363745/1‑90 (MQ=255)                                                                                   cTTGCCGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGAc  <  1:110679/90‑1 (MQ=255)                                                                                        |                                                                                     TGCTCTCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGGAAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCGATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGAC  >  NZ_CP009273/3925931‑3926102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑