Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,964,256	T→C	V255A (GTC→GCC)	wecB →	UDP‑N‑acetylglucosamine 2‑epimerase (non‑hydrolyzing)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,964,256	0	T	C	100.0%	93.7 / NA	27	V255A (GTC→GCC)	wecB	UDP‑N‑acetylglucosamine 2‑epimerase (non‑hydrolyzing)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/8);  total (19/8)

GCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCTGATGAACCACGCC  >  NZ_CP009273/3964169‑3964341                                                                                        |                                                                                      gCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGCCa                                                                                     >  2:77312/1‑90 (MQ=255)     cgcgCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCg                                                                                 <  2:353469/90‑1 (MQ=255)                 cATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCa                                                                     >  2:4499/1‑90 (MQ=255)                         ccacGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGaaaa                                                             >  1:218351/1‑90 (MQ=255)                           acGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAAt                                                           >  2:442944/1‑90 (MQ=255)                             gCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGt                                                         >  1:361361/1‑90 (MQ=255)                                       aTCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTgatc                                               <  1:421475/90‑1 (MQ=255)                                       aTCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTgatc                                               <  1:207134/90‑1 (MQ=255)                                        tCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTgatcg                                              >  1:490189/1‑90 (MQ=255)                                        tCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTgatcg                                              >  2:108214/1‑90 (MQ=255)                                        tCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTgatcg                                              >  2:195281/1‑90 (MQ=255)                                        tCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTgatcg                                              >  2:251719/1‑90 (MQ=255)                                            gATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATcc                                          <  1:249417/90‑1 (MQ=255)                                                gTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAg                                      <  1:201868/90‑1 (MQ=255)                                                 tCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAgg                                     >  1:315182/1‑90 (MQ=255)                                                  cTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGa                                    <  2:494654/90‑1 (MQ=255)                                                          tGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTAc                            >  1:20108/1‑90 (MQ=255)                                                          tGCATCTCAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTAc                            >  2:454197/1‑90 (MQ=255)                                                              tctcAACCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGtt                        >  1:314717/1‑90 (MQ=255)                                                                  aaaccGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTc                    <  2:99071/87‑1 (MQ=255)                                                                   aCCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCt                   >  2:201101/1‑90 (MQ=255)                                                                   aCCCGAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCt                   >  1:488221/1‑90 (MQ=255)                                                                       gAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCt               >  1:499891/1‑90 (MQ=255)                                                                       gAACGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCt               >  2:487469/1‑90 (MQ=255)                                                                        aaCGTCAGAGAACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCtg              >  1:74724/1‑90 (MQ=255)                                                                               gagaACCGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCTGATGAAcc       <  1:201101/90‑1 (MQ=255)                                                                                    ccGGCCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGAATTTACCGTTTGTCTGGCTGATGAACCACGcc  >  1:31455/1‑90 (MQ=255)                                                                                        |                                                                                      GCCACGCGCTGGCAGACATCGCCACCACGCACCAGGACATCCAGATTGTCTATCCGGTGCATCTCAACCCGAACGTCAGAGAACCGGTCAATCGCATTCTGGGGCATGTGAAAAATGTCATTCTGATCGATCCCCAGGAGTATTTACCGTTTGTCTGGCTGATGAACCACGCC  >  NZ_CP009273/3964169‑3964341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑