Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,011,393	A→G	D234G (GAC→GGC)	rmuC →	DNA recombination protein RmuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,011,393	0	A	G	100.0%	124.5 / NA	37	D234G (GAC→GGC)	rmuC	DNA recombination protein RmuC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/18);  total (19/18)

GACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTC  >  NZ_CP009273/4011322‑4011478                                                                        |                                                                                      gACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCa                                                                     <  1:104926/90‑1 (MQ=255) gACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCa                                                                     >  1:138052/1‑90 (MQ=255) gACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCa                                                                     >  2:431723/1‑90 (MQ=255) gACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCa                                                                     >  2:397871/1‑90 (MQ=255) gACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCa                                                                     >  2:35896/1‑90 (MQ=255) gACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCa                                                                     >  2:340051/1‑90 (MQ=255)   cgcgGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGc                                                                   <  2:467192/90‑1 (MQ=255)   cgcgGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGc                                                                   <  1:68863/90‑1 (MQ=255)      gggTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCgg                                                                >  2:441008/1‑90 (MQ=255)      gggTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCgg                                                                >  1:510325/1‑90 (MQ=255)       ggTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGa                                                               <  1:98822/90‑1 (MQ=255)                   ttCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTgcg                                                   >  2:365454/1‑90 (MQ=255)                    tCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTgcgc                                                  >  2:49035/1‑90 (MQ=255)                        gTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGc                                              <  1:12987/90‑1 (MQ=255)                           tGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGc                                           <  1:365454/90‑1 (MQ=255)                             cGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAg                                         <  2:438168/90‑1 (MQ=255)                             cGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAg                                         <  2:124431/90‑1 (MQ=255)                                gAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCGTCGTGGGCCTGCCGCAGGGa                                      <  2:452783/90‑1 (MQ=255)                                 aaGGGTATGAATATGAAACCCAGGTCAGCATCGAGAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGaa                                     <  2:140875/90‑1 (MQ=255)                                      tatgaatatgaaACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCg                                                                 >  1:168823/1‑57 (MQ=255)                                      tatgaatatgaaACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCg                                                                 <  2:168823/57‑1 (MQ=255)                                      tatgaatatgaaACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGAt                                >  1:210553/1‑90 (MQ=255)                                      tatgaatatgaaACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGACATCGTGCGCCTGCCGCAGGGAAAAGAt                                >  2:270214/1‑90 (MQ=255)                                       atgaatatgaaACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGAtg                               >  2:271585/1‑90 (MQ=255)                                             atgaaACCCAGGTCAGCATCGAAAATGGCGCCCGCTcgc                                                                            >  1:32192/1‑39 (MQ=38)                                             atgaaACCCAGGTCAGCATCGAAAATGGCGCCCGCTcgc                                                                            <  2:32192/39‑1 (MQ=38)                                             atgaaACCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGa                         >  1:270265/1‑90 (MQ=255)                                                  aCCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGAc                    <  2:277617/90‑1 (MQ=255)                                                  aCCCAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGAc                    <  2:176457/90‑1 (MQ=255)                                                     cAGGTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGcc                 <  2:210553/90‑1 (MQ=255)                                                        gTCAGCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCaaa              >  2:172982/1‑90 (MQ=255)                                                            gCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGa          >  1:335541/1‑90 (MQ=255)                                                            gCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGa          >  1:193533/1‑90 (MQ=255)                                                            gCATCGAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGa          >  2:128922/1‑90 (MQ=255)                                                                 gAAAATGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTg     <  1:172982/90‑1 (MQ=255)                                                                    aaTGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTc  <  2:270265/90‑1 (MQ=255)                                                                    aaTGGCGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTc  <  2:242689/90‑1 (MQ=255)                                                                        |                                                                                      GACGCGGGTGCTGGAGGCTTCCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTC  >  NZ_CP009273/4011322‑4011478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑