Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	410,572	A→T	L213H (CTC→CAC)	sbcC ←	exonuclease subunit SbcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	410,572	0	A	T	100.0%	26.7 / NA	9	L213H (CTC→CAC)	sbcC	exonuclease subunit SbcC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (6/3);  total (6/3)

GCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGA  >  NZ_CP009273/410497‑410656                                                                            |                                                                                     gcgGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGTGCAACGTGAcgccg                                                                        >  2:193985/1‑90 (MQ=255)              gTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTTTCAGCGATTGCACTTGTTCCGGCGTGTGCAACGTGACGCCGCTGGCCTGCGCtt                                                           >  2:69008/1‑90 (MQ=255)                         tCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGTGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTcc                                                <  2:21750/90‑1 (MQ=255)                              aagtaCCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGTGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGctc                                           <  2:7349/90‑1 (MQ=255)                                      gCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGTGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCggg                                   >  2:447982/1‑90 (MQ=255)                                          aCTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGTGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGa                               >  1:345303/1‑90 (MQ=255)                                            tCGCTGTCAGCGATTGCACTTGTTCCGGCGTGTGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGAtt                             <  1:447982/90‑1 (MQ=255)                                                       gATTGCACTTGTTCCGGCGTGTGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCa                  >  2:107652/1‑90 (MQ=255)                                                                       gCGTGTGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCgaga  >  2:94961/1‑90 (MQ=255)                                                                            |                                                                                     GCGGTAATTAACTGTTTTTCTTCGTCAGTAAGTACCTGCAAACTCGCTGTCAGCGATTGCACTTGTTCCGGCGTGAGCAACGTGACGCCGCTGGCCTGCGCTTGCAGCTTCTCCAGCTCTGTGCGGGCCGATTTGTGCTGCTCAAAAACCATCGCCGAGA  >  NZ_CP009273/410497‑410656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑