Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,014,046	T→C	V154A (GTC→GCC)	ubiB →	ubiquinone biosynthesis regulatory protein kinase UbiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,014,046	0	T	C	100.0%	122.0 / NA	34	V154A (GTC→GCC)	ubiB	ubiquinone biosynthesis regulatory protein kinase UbiB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/6);  total (28/6)

GCTTCTGCTTCTATCGCCCAGGTTCATACCGCGCGATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGTCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATCTACCGTCTGGCTCGCTGGGTGCCGCGTTT  >  NZ_CP009273/4013976‑4014124                                                                       |                                                                               gcttctgcttctATCGCCCAGGTTCATACCGCGCGATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTg                                                             >  2:357473/1‑90 (MQ=255)          tctATCGCCCAGGTTCATACCGCGCGATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGttatt                                                    >  2:433376/1‑90 (MQ=255)                gCCCAGGTTCATACCGCGCGATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCg                                              >  1:72851/1‑90 (MQ=255)                        tCATACCGCGCGATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGaa                                      <  1:206512/90‑1 (MQ=255)                        tCATACCGCGCGATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGaa                                      <  1:309600/90‑1 (MQ=255)                        tCATACCGCGCGATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGaa                                      <  1:130078/90‑1 (MQ=255)                          aTACCGCGCGATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAAc                                    >  2:272782/1‑90 (MQ=255)                               gcgcgATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATcc                                                                           <  1:298059/46‑1 (MQ=255)                               gcgcgATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATcc                                                                           >  2:298059/1‑46 (MQ=255)                               gcgcgATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATc                               >  2:153618/1‑90 (MQ=255)                               gcgcgATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATc                               >  2:488455/1‑90 (MQ=255)                               gcgcgATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATc                               >  2:485327/1‑90 (MQ=255)                               gcgcgATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATc                               >  2:337137/1‑90 (MQ=255)                               gcgcgATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATc                               >  2:152826/1‑90 (MQ=255)                               gcgcgATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATc                               >  2:143375/1‑90 (MQ=255)                               gcgcgATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATc                               >  2:110207/1‑90 (MQ=255)                               gcgcgATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATc                               >  2:120582/1‑90 (MQ=255)                                cgcgATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATCt                              >  1:226767/1‑90 (MQ=255)                                 gcgATTTAAATCGAATGGTAAAGAGGTGGTGAGTAAAGCCATCCGGCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATCTa                             >  2:430167/1‑90 (MQ=255)                                    aTTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATCTACCg                          >  2:482102/1‑90 (MQ=255)                                           tCGAATGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATCTACCGTCTGGCt                   <  1:357473/90‑1 (MQ=255)                                              aaTGGTAAAGAGGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATCTACCGTCTGGCTCGc                >  2:444980/1‑90 (MQ=255)                                                       gagGTGGTGATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATCTACCGTCTGGCTCGCTGGGTGCcg       >  1:139831/1‑90 (MQ=255)                                                          gtggtgATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGaa                                      <  2:442445/56‑1 (MQ=255)                                                          gtggtgATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGaa                                      >  1:442445/1‑56 (MQ=255)                                                            ggtgATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAGACTGATCTACCGTCTGGCTCGCTGGGTGCCGCGttt  >  1:242335/1‑90 (MQ=255)                                                            ggtgATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATCTACCGTCTGGCTCGCTGGGTGCCGCGttt  >  1:429299/1‑90 (MQ=255)                                                            ggtgATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATCTACCGTCTGGCTCGCTGGGTGCCGCGttt  >  1:374878/1‑90 (MQ=255)                                                            ggtgATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATCTACCGTCTGGCTCGCTGGGTGCCGCGttt  >  2:239437/1‑90 (MQ=255)                                                            ggtgATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATCTACCGTCTGGCTCGCTGGGTGCCGCGttt  >  1:255198/1‑90 (MQ=255)                                                            ggtgATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATCTACCGTCTGGCTCGCTGGGTGCCGCGttt  >  2:20109/1‑90 (MQ=255)                                                            ggtgATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATCTACCGTCTGGCTCGCTGGGTGCCGCGttt  >  2:447734/1‑90 (MQ=255)                                                            ggtgATTAAAGCCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATCTACCGTCTGGCTCGCTGGGTGCCGCGttt  >  2:15591/1‑90 (MQ=255)                                                            ggtgATTAAAGCCATCCGCCCGGATATTTTGCCGGGTATTAAAGCGGATCTGAAACTTATCTACCGACTGGCTCGCTGGGTGCCGCGttt  >  2:263938/1‑90 (MQ=255)                                                                       |                                                                               GCTTCTGCTTCTATCGCCCAGGTTCATACCGCGCGATTGAAATCGAATGGTAAAGAGGTGGTGATTAAAGTCATCCGCCCGGATATTTTGCCGGTTATTAAAGCGGATCTGAAACTTATCTACCGTCTGGCTCGCTGGGTGCCGCGTTT  >  NZ_CP009273/4013976‑4014124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑