Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,096,618	T→C	G74G (GGT→GGC)	fieF →	CDF family cation‑efflux transporter FieF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,096,618	0	T	C	100.0%	50.5 / NA	15	G74G (GGT→GGC)	fieF	CDF family cation‑efflux transporter FieF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/7);  total (8/7)

GTCGTTGACGAATTTATTGGTGGTGCGATATTCCCTGCAACCTGCCGACGATAATCACTCGTTTGGTCACGGTAAAGCTGAGTCCCTCGCGGCGCTGGCGCAAAGTATGTTTATCTCCGGTTCGGCACTATTCCTGTTTTTGACGGGTAT  >  NZ_CP009273/4096552‑4096701                                                                   |                                                                                    gtcgtTGACGAATTTATTGGTGGTGCGATATTCCCTGCAACCTGCCGACGATAATCACTCGTTTGGCCACGGTAAAGCTGAGTCCCTcgc                                                              >  1:508299/1‑90 (MQ=255)            aTTTATTGGTGGTGCGATATTCCCTGCAACCTGCCGACGATAATCACTCGTTTGGCCACGGTAAAGCTGAGTCCCTCGCGGCGCTGgcgc                                                   <  1:393781/90‑1 (MQ=255)               tATTGGTGGTGCGATATTCCCTGCAACCTGCCGACGATAATCACTCGTTTGGCCACGGTAAAGCTGAGTCCCTCGCGGCGCTGGCGCaaa                                                <  1:191694/90‑1 (MQ=255)                     tggtgCGATATTCCCTGCAACCTGCCGACGATAATCACTCGTTTGGCCACGGTAAAGCTGAGTCCCTCGCGGCGCTGGCGCaa                                                 <  1:207711/83‑1 (MQ=255)                     tggtgCGATATTCCCTGCAACCTGCCGACGATAATCACTCGTTTGGCCACGGTAAAGCTGAGTCCCTCGCGGCGCTGGCGCaa                                                 >  2:207711/1‑83 (MQ=255)                                 cccTGCAACCTGCCGACGATAATCACTCGTTTGGCCACGGTAAAGCTGAGTCCCTCGCGGCGCTGGCGCAAAGTATGTTTATCTCCGGtt                              >  2:505903/1‑90 (MQ=255)                                 cccTGCAACCTGCCGACGATAATCACTCGTTTGGCCACGGTAAAGCTGAGACCCTCGCGGCGCTGGCGCAAAGTATGTTTATCTCCGGtt                              >  2:487646/1‑90 (MQ=255)                                         ccTGCCGACGATAATCACTCGTTTGGCCACGGTAAAGCTGAGTCCCTCGCGGCGCTGGCGCAAAGTATGTTTATCTCCGGTTCGGCACTa                      <  1:135293/90‑1 (MQ=255)                                           tGCCGACGATAATCACTCGTTTGGCCACGGTAAAGCTGAGTccc                                                                  <  1:64233/44‑1 (MQ=255)                                           tGCCGACGATAATCACTCGTTTGGCCACGGTAAAGCTGAGTccc                                                                  >  2:64233/1‑44 (MQ=255)                                                      aTCACTCGTTTGGCCACGGTAAAGCTGAGTCCCTCGCGGCGCTGGCGCAAAGTATGTTTATCTCCGGTTCGGCACTATTcc                  >  1:498899/1‑81 (MQ=255)                                                      aTCACTCGTTTGGCCACGGTAAAGCTGAGTCCCTCGCGGCGCTGGCGCAAAGTATGTTTATCTCCGGTTCGGCACTATTcc                  <  2:498899/81‑1 (MQ=255)                                                      aTCACTCGTTTGGCCACGGTAAAGCTGAGCCCCTCGCGGCGCTGGCGCAAAGTATGTTTATCTCCGGTTCGGc                          <  1:435931/73‑1 (MQ=255)                                                      aTCACTCGTTTGGCCACGGTAAAGCTGAGCCCCTCGCGGCGCTGGCGCAAAGTATGTTTATCTCCGGTTCGGc                          >  2:435931/1‑73 (MQ=255)                                                             gTTTGGCCACGGTAAAGCTGAGTCCCTCGCGGCGCTGGCGCAAAGTATGTTTATCTCCGGTTCGGCACTATTCCTGTTTTTGACGGGTAt  >  1:266986/1‑90 (MQ=255)                                                                   |                                                                                    GTCGTTGACGAATTTATTGGTGGTGCGATATTCCCTGCAACCTGCCGACGATAATCACTCGTTTGGTCACGGTAAAGCTGAGTCCCTCGCGGCGCTGGCGCAAAGTATGTTTATCTCCGGTTCGGCACTATTCCTGTTTTTGACGGGTAT  >  NZ_CP009273/4096552‑4096701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑