Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,134,706	T→C	S262P (TCC→CCC)	pflD →	formate C‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,134,706	0	T	C	100.0%	83.4 / NA	24	S262P (TCC→CCC)	pflD	formate C‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/12);  total (12/12)

GCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATCATTAACCCAGGGC  >  NZ_CP009273/4134623‑4134783                                                                                    |                                                                              gCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACg                                                                         >  1:336347/1‑90 (MQ=255)      aaCGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGt                                                                    >  2:238648/1‑90 (MQ=255)           gcAACATAAGCCGCAGACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCt                                                               <  2:211047/90‑1 (MQ=255)            cAACATAAGCCGCAGACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTa                                                              <  2:449918/90‑1 (MQ=255)                        cAGACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGgcgc                                                  >  1:313986/1‑90 (MQ=255)                        cAGACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGgcgc                                                  >  1:362477/1‑90 (MQ=255)                        cAGACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGgcgc                                                  >  2:315926/1‑90 (MQ=255)                        cAGACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGgcgc                                                  >  2:294789/1‑90 (MQ=255)                          gACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGGCGCtt                                                <  1:215439/90‑1 (MQ=255)                             gTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGa                                             >  1:376222/1‑90 (MQ=255)                              ttCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGAc                                            <  1:294789/90‑1 (MQ=255)                              ttCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGAc                                            <  2:376222/90‑1 (MQ=255)                              ttCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGAc                                            <  2:362477/90‑1 (MQ=255)                               tCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGAcc                                           <  1:315926/90‑1 (MQ=255)                                cTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCa                                          <  1:238648/90‑1 (MQ=255)                                    cAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGtat                                      >  1:506467/1‑90 (MQ=255)                                      ggCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTatat                                    <  1:354079/90‑1 (MQ=255)                                        cGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGAc                                            <  2:242086/80‑1 (MQ=255)                                        cGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGAc                                            >  1:242086/1‑80 (MQ=255)                                                         tACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGAca                 <  2:258494/90‑1 (MQ=255)                                                         tACATGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGAca                 <  1:455116/90‑1 (MQ=255)                                                            aTGAACATCATTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGAcatca              >  2:379581/1‑90 (MQ=255)                                                                 catcatTCTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATCATTAAc         >  2:493647/1‑90 (MQ=255)                                                                        cTGCAATACGAACCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATCATTAACCCAGGGc  >  1:149552/1‑90 (MQ=255)                                                                                    |                                                                              GCCATAACGCGCAACATAAGCCGCAGACGTTCTGGCAGGCGTGCCAGTTATTCTGGTACATGAACATCATTCTGCAATACGAATCCAACGCCAGTTCGCTATCGTTGGGGCGCTTCGACCAGTATATGTTGCCGTTCTATCAGACATCATTAACCCAGGGC  >  NZ_CP009273/4134623‑4134783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑