| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,136,644 | T→C | T153T (ACT→ACC) | pflC → | [formate‑C‑acetyltransferase]‑activating enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,136,644 | 0 | T | C | 95.2% | 64.6 / ‑3.2 | 21 | T153T (ACT→ACC) | pflC | [formate‑C‑acetyltransferase]‑activating enzyme |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (15/5); total (16/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
GCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGAC > NZ_CP009273/4136562‑4136725 | gcgAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACCGCCGgag > 1:174145/1‑90 (MQ=255) aGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACCGCCGGAGACGCACCAGCCAGCa > 1:222195/1‑90 (MQ=255) aGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACCGCCGGAGACGCACCAGCCAGCa > 1:122780/1‑90 (MQ=255) aGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGGGTGTCATGCGCCATTGAAACCGCCGGAGACGCACCAGCCAGCa > 2:362457/1‑90 (MQ=255) tACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACCGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCt < 1:14184/90‑1 (MQ=255) cccGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTgg > 2:311632/1‑90 (MQ=255) cccGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACCGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTgg > 2:75179/1‑90 (MQ=255) aCAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACCGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATtgtg < 2:77075/90‑1 (MQ=255) gtCATGCGCCATTGAAACCGCCGGAGACGCACCAGCCAGCAAg < 1:291512/43‑1 (MQ=255) gtCATGCGCCATTGAAACCGCCGGAGACGCACCAGCCAGCAAg > 2:291512/1‑43 (MQ=255) tCATGCGCCATTGAAACCGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTaaaa > 1:175325/1‑90 (MQ=255) aTGCGCCATTGAAACCGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAAt > 1:382044/1‑90 (MQ=255) aTGCGCCATTGAAACCGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAAt > 2:517491/1‑90 (MQ=255) aTGCGCCATTGAAACCGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAAt > 1:136895/1‑90 (MQ=255) tGCGCCATTGAAACCGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGc > 2:252496/1‑63 (MQ=255) tGCGCCATTGAAACCGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGc < 1:252496/63‑1 (MQ=255) gcgcCATTGAAACCGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTa > 2:270684/1‑90 (MQ=255) ccATTGAAACCGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATgg > 2:481996/1‑90 (MQ=255) aTTGAAACCGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGAc < 1:362457/90‑1 (MQ=255) aTTGAAACCGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGAc > 2:387642/1‑90 (MQ=255) aTTGAAACCGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGAc > 1:201898/1‑90 (MQ=255) | GCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGTCATGCGCCATTGAAACTGCCGGAGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGATGAAGTGTTGTTCGATTTAAAAATTATGGAC > NZ_CP009273/4136562‑4136725 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |