Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,194,831	A→C	G343G (GGT→GGG)	purD ←	phosphoribosylamine‑‑glycine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,194,831	0	A	C	100.0%	35.9 / NA	11	G343G (GGT→GGG)	purD	phosphoribosylamine‑‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (6/5);  total (6/5)

CGCGTGGAACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGT  >  NZ_CP009273/4194759‑4194906                                                                         |                                                                            cgcgTGGAACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCCCCCGGATATCCACCCGc                                                            >  2:513812/1‑90 (MQ=255)           acTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCCCCCGGATATCCACCCGCAGCCATcacc                                                  <  2:111430/90‑1 (MQ=255)           acTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCCCCCGGATATCCACCCGCAGCCATcacc                                                  <  2:330338/90‑1 (MQ=255)            ttttGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCCCCCGGATATCCACCCGCAGCCATcacca                                                 <  2:391613/89‑1 (MQ=255)            cTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCCCCCGGATATCCACCCGCAGCCATcacca                                                 >  2:135425/1‑90 (MQ=255)            cTTTGCCCCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCCCCCGGATATCCACCCGCAGCCATcacca                                                 <  2:142061/90‑1 (MQ=255)                      tGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCCCCCGGATATCCACCCGCAGCCATCACCACGCCgagaga                                       >  1:523488/1‑90 (MQ=255)                             tcttcCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCCCCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGt                                <  2:522558/90‑1 (MQ=255)                             tcttcCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCCCCCGGATATCCACCCGCAGCCATCACCACCCCCAAATAAGCGCGt                                >  2:452047/1‑90 (MQ=255)                                  cAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCCCCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATc                           >  2:400123/1‑90 (MQ=255)                                                           ccGGTGCGGTAATCCCCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGt  >  2:460937/1‑90 (MQ=255)                                                                         |                                                                            CGCGTGGAACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGT  >  NZ_CP009273/4194759‑4194906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑