Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	411,160	T→C	E17G (GAA→GGA)	sbcC ←	exonuclease subunit SbcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	411,160	0	T	C	100.0%	30.2 / NA	10	E17G (GAA→GGA)	sbcC	exonuclease subunit SbcC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/2);  total (8/2)

GGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCC  >  NZ_CP009273/411078‑411223                                                                                   |                                                                ggtTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATCCGCCttt                                                          <  2:2283/90‑1 (MQ=255)                        cGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATCCGCCTTTTAATGAGTTCAGGTTTTTCAGgc                                   >  1:148900/1‑90 (MQ=255)                                   aaCAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATCCg                                                               >  1:10538/1‑51 (MQ=255)                                   aaCAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATCCg                                                               <  2:10538/51‑1 (MQ=255)                                   aaCAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATCCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTgaga                        >  2:350001/1‑90 (MQ=255)                                   aaCAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATCCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTgaga                        >  2:39180/1‑90 (MQ=255)                                    aCAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATCCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGaa                       >  2:308500/1‑90 (MQ=255)                                    aCAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATCCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGaa                       >  2:343777/1‑90 (MQ=255)                                                      aCGGCTCGCGGGTGAAATCAATCTTCCATCCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTtc     >  1:313732/1‑90 (MQ=255)                                                         gCTCGCGGGTGAAATCAATCTTCCATCCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTcc  >  1:154216/1‑90 (MQ=255)                                                                                   |                                                                GGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCC  >  NZ_CP009273/411078‑411223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑