Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,220,940	A→G	E220G (GAA→GGA)	rluF →	23S rRNA pseudouridine(2604) synthase RluF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,220,940	0	A	G	100.0%	84.0 / NA	24	E220G (GAA→GGA)	rluF	23S rRNA pseudouridine(2604) synthase RluF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/13);  total (11/13)

GCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAA  >  NZ_CP009273/4220871‑4221018                                                                      |                                                                               gCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACcgacg                                                            >  1:30363/1‑90 (MQ=255)  cTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACcgacga                                                           <  1:156528/90‑1 (MQ=255)   tATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGAt                                                          <  1:230062/90‑1 (MQ=255)      gAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAg                                                       <  1:399767/90‑1 (MQ=255)           gAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAAt                                                  >  1:396818/1‑90 (MQ=255)            aaaaaGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATc                                                 <  1:270836/90‑1 (MQ=255)            aaaaaGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATc                                                 <  2:30363/90‑1 (MQ=255)               aaGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGAc                                              <  1:353536/90‑1 (MQ=255)                   tGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGACctct                                          >  1:410286/1‑90 (MQ=255)                   tGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGACctct                                          >  2:222346/1‑90 (MQ=255)                   tGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGACctct                                          >  1:449106/1‑90 (MQ=255)                             gcgcATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCAt                                <  2:204310/90‑1 (MQ=255)                                      gAACGTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGAcc                                             >  2:94496/1‑68 (MQ=255)                                      gAACGTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGAcc                                             <  1:94496/68‑1 (MQ=255)                                        aCGTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCct                     >  1:510719/1‑90 (MQ=255)                                          gTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGAc                                              <  1:387883/63‑1 (MQ=255)                                          gTTAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGAc                                              >  2:387883/1‑63 (MQ=255)                                            tAGCTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTc                 >  1:448706/1‑90 (MQ=255)                                               cTTAAGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGa              <  2:410286/90‑1 (MQ=255)                                                  aaGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGt           >  2:178608/1‑90 (MQ=255)                                                  aaGCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGt           >  1:51054/1‑90 (MQ=255)                                                    gCGGCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTaa         <  2:396818/90‑1 (MQ=255)                                                      ggCATTCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATCCCTCTCCCGAGGTaaaa       <  1:296092/90‑1 (MQ=255)                                                           tCCGCTGGGGGGATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTaa  <  2:129709/90‑1 (MQ=255)                                                                      |                                                                               GCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAA  >  NZ_CP009273/4220871‑4221018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑