Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,233,053	A→G	F131S (TTC→TCC)	malG ←	maltose ABC transporter permease MalG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,233,053	0	A	G	100.0%	71.9 / NA	21	F131S (TTC→TCC)	malG	maltose ABC transporter permease MalG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/9);  total (12/9)

AGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGACCAGTGAAAGTACTGCCGGGAACATCTGGAAAATCAGCATTCCTTTCAGCAGCGTCGCTTTGCCTGGAAAGCGCATACGGGCGAAAGCGTAGGCGCAGGTGGTGG  >  NZ_CP009273/4232978‑4233137                                                                            |                                                                                     aGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGACCAGTGAAAGTACTGCCGGGGACATCTGGAAAATc                                                                        >  1:441468/1‑90 (MQ=255)   gCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGACCAGTGAAAGTACTGCCGGGGACATCTGGAAAATCAg                                                                      >  1:43268/1‑90 (MQ=255)      aatgaatgGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGACCAGTGAAAGTACTGCCGGGGACATCTGGAAAATCAGCAt                                                                   <  1:18350/90‑1 (MQ=255)        tgaatgGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGACCAGTGAAAGTACTGCCGGGGACATCTGGAAAATCAGCATTc                                                                 <  2:420295/90‑1 (MQ=255)                atgTACTCACCCAGACGATCAAACAACGCATACAACGCGACCAGTGAAAGTACTGCCGGGGACATCTGGAAAATCAGCATTCCTTTcagc                                                         >  1:303087/1‑90 (MQ=255)                atgTACTCACCCAGACGATCAAACAACGCATACAACGCGACCAGTGAAAGTACTGCCGGGGACATCTGGAAAATCAGCATTCCTTTcagc                                                         >  2:307866/1‑90 (MQ=255)                    aCTCACCCAGACGATCAAACAACGCATACAACGCGACCAGTGAAAGTACTGCCGGGGACATCTGGAAAATCAGCATTCCTTTCAGCAGCg                                                     >  1:451494/1‑90 (MQ=255)                       cACCCAGACGATCAAACAACGCATACAACGCGACCAGTGAAAGTACTGCCGGGGACATCTGGAAAATCAGCATTCCTTTCAGCAGCGTCg                                                  >  1:449267/1‑90 (MQ=255)                            aGACGATCAAACAACGCATACAACGCGACCAGTGAAAGTACTGCCGGGGACATCTGGAAAATCAGCATTCCTTTCAGCAGCGTCGCTTTg                                             >  2:419586/1‑90 (MQ=255)                                  tCAAACAACGCATACAACGCGACCAGTGAAAGTACTGCCGGGGACATCTGGAAAATCAGCATTCCTTTCAGCAGCGTCGCTTTGCCTGGa                                       >  1:403667/1‑90 (MQ=255)                                           gCATACAACGCGACCAGTGAAAGTACTGCCGGGGACATCTGGAAAATCAGCATTc                                                                 >  2:439664/1‑55 (MQ=255)                                           gCATACAACGCGACCAGTGAAAGTACTGCCGGGGACATCTGGAAAATCAGCATTc                                                                 <  1:439664/55‑1 (MQ=255)                                                 aaCGCGACCAGTGAAAGTACTGCCGGGGACATCTGGAAAATCAGCATTCCTTTCAGCAGCGTCGCTTTGCCTGGAAAGCGCATACGGGCg                        >  2:436318/1‑90 (MQ=255)                                                    gcgACCAGTGAAAGTACTGCCGGGGACATCTGGAAAATCAGCATTCCTTTCAGCAGCGTCGCTTTGCCTGGAAAGCGCATACGGGCGaaa                     <  1:327874/90‑1 (MQ=255)                                                          aGTGAAAGTACTGCCGGGGACATCTGGAAAATCAGCATTCCTTTCAGCAGCGTCGCTTTGCCTGGAAAGCGCATACGGGCGAAAGCGTAg               >  2:185437/1‑90 (MQ=255)                                                               aaGTACTGCCGGGGACATCTGGAAAATCAGCATTCCTTTCAGCAGCGTCGCTTTGCCTGGAAAGCGCATACGGGCGAAAGCGTAGGCGCa          <  2:178294/90‑1 (MQ=255)                                                               aaGTACTGCCGGGGACATCTGGAAAATCAGCATTCCTTTCAGCAGCGTCGCTTTGCCTGGAAAGCGCATACGGGCGAAAGCGTAGGCGCa          <  2:213567/90‑1 (MQ=255)                                                               aaGTACTGCCGGGGACATCTGGAAAATCAGCATTCCTTTCAGCAGCGTCGCTTTGCCTGGAAAGCGCATACGGGCGAAAGCGTAGGCGCa          <  1:95269/90‑1 (MQ=255)                                                               aaGTACTGCCGGGGACATCTGGAAAATCAGCATTCCTTTCAGCAGCGTCGCTTTGCCTGGAAAGCGCATACGGGCGAAAGCGTAGGCGCa          <  2:43268/90‑1 (MQ=255)                                                                     tGCCGGGGACATCTGGAAAATCAGCATTCCTTTCAGCAGCGTCGCTTTGCCTGGAAAGCGCATACGGGCGAAAGCGTAGGCGCAggtggt    >  2:408519/1‑90 (MQ=255)                                                                       ccGGGGACATCTGGAAAATCAGCATTCCTTTCAGCAGCGTCGCTTTGCCTGGAAAGCGCATACGGGCGAAAGCGTAGGCGCAGGtggtgg  <  2:136249/90‑1 (MQ=255)                                                                            |                                                                                     AGGCCAATGAATGGAATGTACTCACCCAGACGATCAAACAACGCATACAACGCGACCAGTGAAAGTACTGCCGGGAACATCTGGAAAATCAGCATTCCTTTCAGCAGCGTCGCTTTGCCTGGAAAGCGCATACGGGCGAAAGCGTAGGCGCAGGTGGTGG  >  NZ_CP009273/4232978‑4233137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑