Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,279,722	C→T	L184F (CTT→TTT)	nrfB →	cytochrome c nitrite reductase pentaheme subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,279,722	0	C	T	100.0%	91.2 / NA	24	L184F (CTT→TTT)	nrfB	cytochrome c nitrite reductase pentaheme subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/11);  total (13/11)

GTGACAAAGGACGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCG  >  NZ_CP009273/4279636‑4279806                                                                                       |                                                                                     gTGACAAAGGACGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGTTTa                                                                                   <  2:10529/90‑1 (MQ=255)  tGACAAAGGACGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGTTTaa                                                                                  <  1:71927/90‑1 (MQ=255)     cAAAGGACGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGTTTAAGGa                                                                               >  1:246368/1‑90 (MQ=255)     cAAAGGACGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGTTTAAGGa                                                                               >  2:117991/1‑90 (MQ=255)       aaGGACGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGTTTAAGGagc                                                                             <  2:246368/90‑1 (MQ=255)       aaGGACGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGTTTAAGGagc                                                                             <  1:92370/90‑1 (MQ=255)            cGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGTTTAAGGAGCAGCCa                                                                        <  1:105915/90‑1 (MQ=255)            cGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGTTTAAGGAGCAGCCa                                                                        <  2:469634/90‑1 (MQ=255)              gATTAAGATTTGCGTCAATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGTTTAAGGAGCAGCCATg                                                                      >  1:58887/1‑90 (MQ=255)                       ttGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGTTTAAGGAGCAGCCATGACCTGGtct                                                             >  1:190047/1‑90 (MQ=255)                        tGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGTTTAAGGAGCAGCCATGACCTGGtctc                                                            >  1:493373/1‑90 (MQ=255)                        tGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGCCGTCTGTTCCGTTGTTTAAGGAGCAGCCATGACCTGGtctc                                                            >  1:394244/1‑90 (MQ=255)                        ggCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGTTTAAGGAGCAGCCATGACCTGGcctc                                                            >  2:334293/2‑90 (MQ=255)                                             cAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGTTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACcggcg                                       >  1:460241/1‑90 (MQ=255)                                               gcgcACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGTTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACcggcgtc                                     >  2:183587/1‑90 (MQ=255)                                                cgcACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGTTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACcggcgtcg                                    <  1:373780/90‑1 (MQ=255)                                                cgcACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGTTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACcggcgtcg                                    <  1:231014/90‑1 (MQ=255)                                                         ccGAACTTTAACCCGGCGTCTGTTCCGTTGTTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTgg                           >  1:197614/1‑90 (MQ=255)                                                          cGAACTTTAACCCGGCGTCTGTTCCGTTGTTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGc                          <  2:229060/90‑1 (MQ=255)                                                                  aaCCCGGCGTCTGTTCCGTTGTTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCa                  <  1:90214/90‑1 (MQ=255)                                                                      cGGCGTCTGTTCCGTTGTTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCgg              <  2:354236/90‑1 (MQ=255)                                                                        gCGTCTGTTCCGTTGTTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCa            >  1:458160/1‑90 (MQ=255)                                                                        gCGTCTGTTCCGTTGTTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCa            >  1:383370/1‑90 (MQ=255)                                                                                  cGTTGTTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGgcg  >  2:510615/1‑90 (MQ=255)                                                                                       |                                                                                     GTGACAAAGGACGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAACTTTAACCCGGCGTCTGTTCCGTTGCTTAAGGAGCAGCCATGACCTGGTCTCGTCGCCAGTTTCTCACCGGCGTCGGCGTGCTGGCAGCCGTCAGCGGCACCGCAGGGCG  >  NZ_CP009273/4279636‑4279806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑