| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,325,912 | T→A | I313F (ATT→TTT) | adiC ← | arginine/agmatine antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,325,912 | 0 | T | A | 92.9% | 39.6 / NA | 14 | I313F (ATT→TTT) | adiC | arginine/agmatine antiporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (9/4); minor base C (0/1); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGAGCTGGAAGATGGTCATCAAAATACCGACGATAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGTTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAA > NZ_CP009273/4325829‑4325994 | gctgAGCTGGAAGATGGTCATCAAAATACCGACGATAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAAACGGTgg > 2:555/1‑90 (MQ=255) ggTCATCAAAATACCGACGATAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAAACGGTGGGAACAGTCCGTCATc > 1:9795/1‑90 (MQ=255) ggTCATCAAAATACCGACGATAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAAACGGTGGGAACAGTCCGTCATc > 2:99883/1‑90 (MQ=255) gacgaTAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAAACGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGc > 1:44197/1‑90 (MQ=255) gacgaTAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAAACGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGc > 2:51089/1‑90 (MQ=255) ccccccccgccccgGGCGTCCCCGCTTTATTTACACGGGCAAAAAACGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGTTTGAc < 2:3303/86‑1 (MQ=255) ccGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAAACGGTg > 1:183712/1‑45 (MQ=255) ccGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAAACGGTg < 2:183712/45‑1 (MQ=255) ccACTGGCGTACCCGCTTTATTTACACGGGCAAAAAACGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGTTTGACCCGCCAGCa < 1:555/90‑1 (MQ=255) aCCCGCTTTATTTACACGGGCAAAAAACGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGTTTGACCCGCCAGCAACGTCCAgcc > 2:129147/1‑90 (MQ=255) aCCCGCTTTATTTACACGGGCAAAAAACGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGTTTGACCCGCCAGCAACGTCCAgcc > 2:76333/1‑90 (MQ=255) gggAAAAAACCGGGGGAAACAGCCCGCCACCGGCGGCGGTTTTCGCCTTTTGCCCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGc < 2:175917/90‑1 (MQ=255) gCAAAAAACGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGTTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCaa < 1:198742/90‑1 (MQ=255) gCAAAAAACGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGTTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCaa > 1:438634/1‑90 (MQ=255) | GCTGAGCTGGAAGATGGTCATCAAAATACCGACGATAATCAGCCCCGCCACTGGCGTACCCGCTTTATTTACACGGGCAAAAATCGGTGGGAACAGTCCGTCATCGGCAGCGGCTTTCGCCGTTTGACCCGCCAGCAACGTCCAGCCGCCCAGTGAACCTAAGCAA > NZ_CP009273/4325829‑4325994 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |