Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	28,032	T→C	F247S (TTC→TCC)	rihC →	ribonucleoside hydrolase RihC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	28,032	0	T	C	100.0%	76.1 / NA	22	F247S (TTC→TCC)	rihC	ribonucleoside hydrolase RihC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/8);  total (14/8)

TAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGCGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGC  >  NZ_CP009273/27946‑28114                                                                                       |                                                                                   tAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTCCAc                                                                                 <  2:456352/90‑1 (MQ=255)      aCTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTCCACTCTCa                                                                            >  1:170746/1‑90 (MQ=255)      aCTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTCCACTCTCa                                                                            >  1:152755/1‑90 (MQ=255)        tACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTCCACTCTCaaa                                                                          >  1:356572/1‑90 (MQ=255)        tACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTCCACTCTCaaa                                                                          >  1:347759/1‑90 (MQ=255)         aCCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTCCACTCTCAAAc                                                                         <  2:152755/90‑1 (MQ=255)            gTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTCCACTCTCAAACCCt                                                                      <  2:68923/90‑1 (MQ=255)            gTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGGGCGCCCGGACCTGTCCACTCTCAAACCCt                                                                      >  2:249550/1‑90 (MQ=255)                       aTGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTCCACTCTCAAACCCTGTTTTGTGGCa                                                           <  2:285769/90‑1 (MQ=255)                       aTGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTCCACTCTCAAACCCTGTTTTGTGGCa                                                           >  2:308770/1‑90 (MQ=255)                            aaGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTCCACTCTCAAACCCTGTTTTGTGGCa                                                           >  1:298885/1‑85 (MQ=255)                            aaGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTCCACTCTCAAACCCTGTTTTGTGGCa                                                           <  2:298885/85‑1 (MQ=255)                                ggCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTCCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACt                                                  >  1:374681/1‑90 (MQ=255)                                                   tctGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTCCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGCGAATTTACCTCAg                               <  1:222554/90‑1 (MQ=255)                                                        gcCATCGCCTGGCTGGTGCGCCCGGACCTGTCCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGCGAATTTACCTCAGGCacg                          <  2:459244/90‑1 (MQ=255)                                                          cATCGCCTGGCTGGTGCGCCCGGACCTGTCCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGCGAATTTACCTCAGGCacgac                        >  1:294310/1‑90 (MQ=255)                                                          cATCGCCTGGCTGGTGCGCCCGGACCTGTCCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGCGAATTTACCTCAGGCacgac                        >  2:308961/1‑90 (MQ=255)                                                           aTCGCCTGGCTGGTGCGCCCGGACCTGTCCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGCGAATTTACCTCAGGCacgacg                       >  1:457293/1‑90 (MQ=255)                                                                       gTGCGCCCGGACCTGTCCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGCGAATTTACCTCAGGCACGACGGTGGTTGATATc           >  1:452878/1‑90 (MQ=255)                                                                           gcCCGGACCTGTCCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGCGAATTTACCTCAGGCACGACggt                     >  1:477388/1‑76 (MQ=255)                                                                           gcCCGGACCTGTCCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGCGAATTTACCTCAGGCACGACggt                     <  2:477388/76‑1 (MQ=255)                                                                                gACCTGTCCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGCGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGc  >  2:184162/1‑90 (MQ=255)                                                                                       |                                                                                   TAGCCACTACCGTAGCGGCAGTATGCAAAGCGGCTTGCGAATGCACGATCTCTGCGCCATCGCCTGGCTGGTGCGCCCGGACCTGTTCACTCTCAAACCCTGTTTTGTGGCAGTGGAAACTCAGGGCGAATTTACCTCAGGCACGACGGTGGTTGATATCGACGGTTGC  >  NZ_CP009273/27946‑28114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑