Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,329,995	A→G	I115T (ATT→ACT)	adiA ←	arginine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,329,995	0	A	G	100.0%	33.6 / NA	11	I115T (ATT→ACT)	adiA	arginine decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/7);  total (4/7)

CTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCA  >  NZ_CP009273/4329908‑4330067                                                                                        |                                                                         cTGAACAGTGGCGGCAACAGCTGCTGGCGGGAGCGGGGCATCGCGGCAACGGCGCGGCCGGCGATAAAGTCGGCGGGATCTTTCAGAGTc                                                                        >  2:81120/1‑90 (MQ=255)    aaCAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAGTCCAg                                                                     <  1:81120/90‑1 (MQ=255)                  cAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAGTCCAGGCGAATTCATCGAc                                                       >  1:219911/1‑90 (MQ=255)                  cAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAGTCCAGGCGAATTCATCGAc                                                       <  2:45273/90‑1 (MQ=255)                    gctgctGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAGTCCAGGCGAATTCATCGACaa                                                     <  2:203584/90‑1 (MQ=255)                                aGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAGTCCAGGCGAATTCATCGACAAGCTCCAGCAgga                                         <  1:94733/90‑2 (MQ=255)                                    ggTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAGTCCAGGCGAATTCATCGACAAGCTCCAGCAGGTcgcg                                     >  2:124000/1‑90 (MQ=255)                                                                  aaaGTCGGCGGTATCTTCCAGAGTCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATc       <  1:115381/90‑1 (MQ=255)                                                                  aaaGTCGGCGGTATCTTCCAGAGTCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATc       <  2:80344/90‑1 (MQ=255)                                                                       cggcggTATCTTCCAGAGTCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCa  <  2:473791/90‑1 (MQ=255)                                                                       cggcggTATCTTCCAGAGTCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCa  >  2:51753/1‑90 (MQ=255)                                                                                        |                                                                         CTGAACAGTGGCGGCAACAGCTGCTGGCGGTAGCGGGTCATCGCGGCAACGGCGCGTCCGGCGATAAAGTCGGCGGTATCTTCCAGAATCCAGGCGAATTCATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCA  >  NZ_CP009273/4329908‑4330067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑