Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,347,351	T→G	T362P (ACC→CCC)	cadA ←	lysine decarboxylase CadA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,347,351	0	T	G	100.0%	87.7 / NA	25	T362P (ACC→CCC)	cadA	lysine decarboxylase CadA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (13/12);  total (13/12)

AAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAA  >  NZ_CP009273/4347269‑4347430                                                                                   |                                                                                aaGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTaa                                                                          <  1:127503/90‑1 (MQ=255)     tttCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCa                                                                      >  1:1383/1‑90 (MQ=255)        cttcGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACtt                                                                   <  2:79638/90‑1 (MQ=255)        cttcGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACtt                                                                   <  2:1383/90‑1 (MQ=255)          tcGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTc                                                                 <  1:201813/90‑1 (MQ=255)             tttACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTCCCt                                                              >  1:320740/1‑90 (MQ=255)                         ttAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAGTCACTTTCCCTTCTACACGGCCa                                                  <  2:480488/90‑1 (MQ=255)                         ttAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTCCCTTCTACACGGCCa                                                  <  2:480489/90‑1 (MQ=255)                                ggATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTc                                                                 <  1:456250/68‑1 (MQ=255)                                ggATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTc                                                                 >  2:456250/1‑68 (MQ=255)                                ggATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCa                                           >  2:101712/1‑90 (MQ=255)                                ggATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCa                                           >  2:292149/1‑90 (MQ=255)                                   tCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATAc                                        >  2:164414/1‑90 (MQ=255)                                           gCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTa                                >  2:325658/1‑90 (MQ=255)                                           gCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTa                                >  2:305016/1‑90 (MQ=255)                                           gCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTa                                >  2:31923/1‑90 (MQ=255)                                               gagagaACGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCtt                            >  2:325755/1‑90 (MQ=255)                                                    aaCGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCAt                                          >  2:374856/1‑71 (MQ=255)                                                    aaCGCCGCCAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCAt                                          <  1:374856/71‑1 (MQ=255)                                                       gccgccAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATc                    <  2:175632/90‑1 (MQ=255)                                                        ccgccAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCg                   >  2:203387/1‑90 (MQ=255)                                                           ccAGCAGTTTGTGAGTGGACTGGGGTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTg                <  1:14823/90‑1 (MQ=255)                                                                  tttGTGAGTGGACTGGGGTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGtt         <  2:139032/90‑1 (MQ=255)                                                                  tttGTGAGTGGACTGGGGTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGtt         >  1:40113/1‑90 (MQ=255)                                                                         gTGGACTGGGGTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTaa  <  2:142711/90‑1 (MQ=255)                                                                                   |                                                                                AAGGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAA  >  NZ_CP009273/4347269‑4347430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑