Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,379,477	T→C	E234G (GAA→GGA)	asd ←	archaetidylserine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,379,477	0	T	C	100.0%	88.7 / NA	25	E234G (GAA→GGA)	asd	archaetidylserine decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/14);  total (11/14)

GGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTGCCCGCCCAGACC  >  NZ_CP009273/4379393‑4379545                                                                                     |                                                                     ggTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCg                                                                 >  1:164234/1‑90 (MQ=255)   tGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGc                                                               <  2:203746/90‑1 (MQ=255)            gTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGt                                                      >  1:45762/1‑90 (MQ=255)                 tAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAgc                                                 <  2:177952/90‑1 (MQ=255)                    cGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCt                                              <  2:409067/90‑1 (MQ=255)                      gTGGAACCGAGTTTAAAGCGACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCa                                                              <  2:82294/72‑1 (MQ=255)                      gTGGAACCGAGTTTAAAGCGACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCa                                                              >  1:82294/1‑72 (MQ=255)                        ggAACCGAGTTTAAAGCGACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCTtgat                                          <  2:45762/90‑1 (MQ=255)                              gAGTTTAAAGCGACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCTTGATGATAcc                                    >  2:177972/1‑90 (MQ=255)                                        cGACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCgcggc                          <  1:309975/90‑1 (MQ=255)                                        cGACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCgcggc                          <  2:328597/90‑1 (MQ=255)                                         gACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCgcggcg                         >  1:357914/1‑90 (MQ=255)                                         gACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCgcggcg                         >  2:208511/1‑90 (MQ=255)                                          aCCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCgcggcgg                        <  2:357914/90‑1 (MQ=255)                                          aCCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCgcggc                          >  2:194583/1‑88 (MQ=255)                                          aCCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCgcggc                          <  1:194583/88‑1 (MQ=255)                                                ttCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAAt                  <  2:354166/90‑1 (MQ=255)                                                         tttCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTgcccgc         >  2:278975/1‑90 (MQ=255)                                                          ttCAGCAGTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTgcccgcc        >  1:367946/1‑90 (MQ=255)                                                               cagTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTGCCCGCCCAGAc   <  1:446652/90‑1 (MQ=255)                                                                agTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTGCCCGCCCAGAcc  <  1:208511/90‑1 (MQ=255)                                                                 gTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCt                                              >  2:265168/1‑45 (MQ=255)                                                                 gTGCCACAGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCt                                              <  1:265168/45‑1 (MQ=255)                                                                      acaGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCgcggc                          >  1:331407/1‑60 (MQ=255)                                                                      acaGAACCGTCGTTTCCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCgcggc                          <  2:331407/60‑1 (MQ=255)                                                                                     |                                                                     GGTGCAAACAGGTTGATAACGGTGGAACCGAGTTTAAAGCGACCCATTTCCTGGCCTTTCAGCAGTGCCACAGAACCGTCGTTTTCCCCGGCAGGCCAGGTCCAGCGCTTGATGATACCTTCGCGCGGCGGCGTAATGGTGCCCGCCCAGACC  >  NZ_CP009273/4379393‑4379545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑