| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,423,096 | A→G | V249A (GTT→GCT) | qorB ← | NAD(P)H:quinone oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,423,096 | 0 | A | G | 86.7% | 33.9 / ‑2.9 | 15 | V249A (GTT→GCT) | qorB | NAD(P)H:quinone oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (10/3); total (11/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGAT > NZ_CP009273/4423014‑4423180 | ttCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAGCGTCAGa > 1:134014/1‑90 (MQ=255)ttCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAGCGTCAGa > 1:184821/1‑90 (MQ=255) gTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAGCGTCAGAATCCGCCAGCATATCCGCCAg < 1:63262/90‑1 (MQ=255) ggCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAGCGTCAGAATCCGCCAGCATATCCGCCAGTc > 1:263817/1‑90 (MQ=255) ggCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAGCGTCAGAATCCGCCAGCATATCCGCCAGTc > 2:291977/1‑90 (MQ=255) gCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTcc > 2:365302/1‑90 (MQ=255) aatTTGCTAAGTTTTTTGCTGTCATCAAACAGCCCGCCTTTCGATGCGCCAGCGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGc < 2:62459/90‑1 (MQ=255) atTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAGCGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCa < 2:134014/90‑1 (MQ=255) gtgtTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGc < 1:365302/90‑1 (MQ=255) gTCATCAAACAGACCGCCTTTCGATGCGCCAGCGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGc > 1:164700/1‑90 (MQ=255) gTCATCAAACAGACCGCCTTTCGATGCGCCAGCGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGc > 1:265837/1‑90 (MQ=255) aGACCGCCTTTCGATGCGCCAGCGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGaaa > 2:2592/1‑90 (MQ=255) gACCGCCTTTCGATGCGCCAGCGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAAt > 1:435525/1‑90 (MQ=255) cGATGCGCCAGCGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCt > 1:416167/1‑90 (MQ=255) cgcCAGCGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGAt > 2:243332/1‑90 (MQ=255) | TTCGGCTAACGTTGTCGTTGGGTGGCCAATCAATTTGCTAAGTGTTTTGCTGTCATCAAACAGACCGCCTTTCGATGCGCCAACGTCAGAATCCGCCAGCATATCCGCCAGTCCGTCGGGCAGTCCGACGCTTTTCAGTGCCGCGGCGAAATCGGCTTCGCTCAGAT > NZ_CP009273/4423014‑4423180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |