Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,471,160	T→C	D832G (GAT→GGT)	valS ←	valine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,471,160	0	T	C	100.0%	57.6 / NA	17	D832G (GAT→GGT)	valS	valine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/11);  total (6/11)

CGGCAGGCAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCATCCGCGCTGCAACCACGCAGCAGCAGCTCCAGCGGTTTGCCCGGCGCGATGTTCATTTCTGCACGGATGTTACGTACCGC  >  NZ_CP009273/4471080‑4471239                                                                                 |                                                                                cGGCAGGCAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCACCCGCGCTGc                                                                        >  1:182509/1‑90 (MQ=255)      ggcagCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCACCCGCGCTGCAACCa                                                                   >  1:132329/1‑90 (MQ=255)       gcagcaCGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCACCCGCGCTGCAACCAc                                                                  <  2:213763/90‑1 (MQ=255)          gcaCGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCACCCGCGCTGCAACCACgca                                                               <  2:319330/90‑1 (MQ=255)             cGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCACCCGCGCTGCAACCACgcagca                                                            >  2:514298/1‑90 (MQ=255)                tGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCACCCGCGCt                                                                          <  2:87528/73‑1 (MQ=255)                tGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCACCCGCGCt                                                                          >  1:87528/1‑73 (MQ=255)                    aCTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCACCCGCGCTGCAACCACGCAGCAGCAGCTc                                                     >  2:78230/1‑90 (MQ=255)                       ttCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCACCCGCGCTGCAACCACGCAGCAGCAGCTCCAg                                                  >  1:310806/1‑90 (MQ=255)                       ttCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCACCCGCGCTGCAACCACGCAGCAGCAGCTCCAg                                                  <  1:346780/90‑1 (MQ=255)                                        tttGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCACCCGCGCTGCAACCACGCAGCAGCAGCTCCAGCGGTTTGCCCGGCGCGa                                 <  1:156190/90‑1 (MQ=255)                                        tttGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCACCCGCGCTGCAACCACGCAGCAGCAGCTCCAGCGGTTTGCCCGGCGCGa                                 <  2:147026/90‑1 (MQ=255)                                           gCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCACCCGCGCTGCAACCACGCAGCAGCAGCTCCAGCGGTTTGCCCGGCGCGATGt                              <  1:432348/90‑1 (MQ=255)                                             aGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCACCCGCGCTGCAACCACGCAGCAGCAGCTCCAGCGGTTTGCCCGGCGCGATGTTc                            <  1:367863/90‑1 (MQ=255)                                                          tttCATTTACGCGACGTTCTGCACCCGCGCTGCAACCACGCAGCAGCAGCTCCAGCGGTTTGCCCGGCGCGATGTTCATTTCTGCACGGa               <  2:139370/90‑1 (MQ=255)                                                          tttCATTTACGCGACGTTCTGCACCCGCGCTGCAACCACGCAGCAGCAGCTCCAGCGGTTTGCCCGGCGCGATGTTCATTTCTGCACGGa               <  1:165410/90‑1 (MQ=255)                                                                       aCGTTCTGCACCCGCGCTGCAACCACGCAGCAGCAGCTCCAGCGGTTTGCCCGGCGCGATGTTCATTTCTGCACGGATGTTACGTACCGc  <  1:385824/90‑1 (MQ=255)                                                                                 |                                                                                CGGCAGGCAGCACGGTGATACTTTCCAGACGCGCCAGGGTTTGCAGGAAGCCACGGTTTTCATTTACGCGACGTTCTGCATCCGCGCTGCAACCACGCAGCAGCAGCTCCAGCGGTTTGCCCGGCGCGATGTTCATTTCTGCACGGATGTTACGTACCGC  >  NZ_CP009273/4471080‑4471239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑