Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,482,153	T→C	I64V (ATC→GTC)	idnT ←	gnt‑II system L‑idonate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,482,153	0	T	C	100.0%	108.5 / NA	31	I64V (ATC→GTC)	idnT	gnt‑II system L‑idonate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/18);  total (13/18)

CAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGC  >  NZ_CP009273/4482079‑4482231                                                                           |                                                                               cAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCccgcc                                                                 >  1:448514/1‑90 (MQ=255)    cGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAg                                                              <  2:25038/90‑1 (MQ=255)         tGGCGATACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGtgc                                                         <  1:384799/90‑1 (MQ=255)             gagaCGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTACCAGCCCGCCGAGCGTGCTGcc                                                     >  2:378046/4‑90 (MQ=255)             gATACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGcc                                                     >  2:187301/1‑90 (MQ=255)               tACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGACCCGAAACCGAGGACCATTGCCAGCCCGCCGAGAGTGCTGCCGa                                                   <  1:255603/90‑1 (MQ=255)                  gTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATAc                                                <  2:158451/90‑1 (MQ=255)                    tgtgCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATAc                                                >  2:428592/1‑88 (MQ=255)                    tgtgCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATAc                                                <  1:428592/88‑1 (MQ=255)                    tgtgCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCa                                              >  1:175073/1‑90 (MQ=255)                    tgtgCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCa                                              <  1:67549/90‑1 (MQ=255)                    tgtgCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCa                                              <  1:187301/90‑1 (MQ=255)                      tgCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCAtt                                            <  1:478105/90‑1 (MQ=255)                       gCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCAttt                                           <  2:92010/90‑1 (MQ=255)                       gCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCAttt                                           <  1:20540/90‑1 (MQ=255)                           cACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGta                                       >  2:22/1‑90 (MQ=255)                              ccGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATag                                    <  2:361839/90‑1 (MQ=255)                              ccGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATag                                    <  1:473271/90‑1 (MQ=255)                                  aTCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGta                                       >  2:125882/1‑83 (MQ=255)                                  aTCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGta                                       <  1:125882/83‑1 (MQ=255)                                        aaTCAGCTTGCCTAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGAc                          <  2:174129/90‑1 (MQ=255)                                                    tAACATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGcatc              >  2:279670/1‑90 (MQ=255)                                                      aCATGGCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGcatcca            >  1:221154/1‑90 (MQ=255)                                                          ggCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGcc                      >  1:28178/1‑76 (MQ=255)                                                          ggCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGcc                      <  2:28178/76‑1 (MQ=255)                                                           gCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGAc                          >  1:358791/1‑71 (MQ=255)                                                           gCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGAc                          <  2:358791/71‑1 (MQ=255)                                                           gCCCCGAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCt       >  1:445191/1‑90 (MQ=255)                                                                gAAACCGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGc  <  2:175073/90‑1 (MQ=255)                                                                    ccGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATag                                    <  1:519537/52‑1 (MQ=255)                                                                    ccGAGGACCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATag                                    >  2:519537/1‑52 (MQ=255)                                                                           |                                                                               CAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGC  >  NZ_CP009273/4482079‑4482231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑