| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 436,468 | A→G | T30T (ACT→ACC) | ispA ← | (2E,6E)‑farnesyl diphosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 436,468 | 0 | A | G | 96.2% | 86.4 / ‑4.4 | 26 | T30T (ACT→ACC) | ispA | (2E,6E)‑farnesyl diphosphate synthase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (17/8); total (18/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGT > NZ_CP009273/436386‑436553 | tatGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGGGTGTTCt < 1:69566/90‑1 (MQ=255) ccGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGGGTGTTCTGAAAg < 1:122995/90‑1 (MQ=255) ggTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGGGTGTTCTGAAAggg > 1:360374/1‑90 (MQ=255) gTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGGGTGTTCTGAAAGGGc > 1:323411/1‑90 (MQ=255) ggCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGGGTGTTCTGAAAGGGCAg > 1:442997/1‑90 (MQ=255) cATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTg > 1:169691/1‑90 (MQ=255) ccAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGa > 1:34451/1‑90 (MQ=255) cAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGAt < 2:469098/90‑1 (MQ=255) gcgcTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAgcgc < 2:360374/90‑1 (MQ=255) cTAATAATGCGCCATACTGCATGGTTTCGACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCt > 2:33560/1‑90 (MQ=255) taataaTGCGCCATACTGCATGGTTTCGACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTg > 2:117270/1‑90 (MQ=255) atGCGCCATACTGCATGGTTTCGACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTaa > 1:81097/1‑90 (MQ=255) cgcCATACTGCATGGTTTCGACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGc > 1:284671/1‑90 (MQ=255) cgcCATACTGCATGGTTTCGACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGc > 2:519480/1‑90 (MQ=255) gcCATACTGCATGGTTTCGACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCa < 2:240942/90‑1 (MQ=255) gCATGGTTTCGACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGa > 2:234340/1‑90 (MQ=255) aTGGTTTCGACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGt > 1:432837/1‑90 (MQ=255) gTTTCGACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTc < 1:65279/51‑1 (MQ=255) gTTTCGACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTc > 2:65279/1‑51 (MQ=255) gTTTCGACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTtgc > 2:371286/1‑90 (MQ=255) ttCGACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTtgctg < 1:33560/90‑1 (MQ=255) gACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCgg > 2:341418/1‑90 (MQ=255) gACCACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCgg > 2:353344/1‑90 (MQ=255) ccACGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGaa < 1:116071/90‑1 (MQ=255) aCGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAg > 1:405729/1‑90 (MQ=255) cGGGGGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGt > 1:490268/1‑90 (MQ=255) | TATGACCGGTGGCATAAACCAGGAAAGGTCGCAGGCGCTTACCACCTAATAATGCGCCATACTGCATGGTTTCGACCACGGGAGTGTTCTGAAAGGGCAGTGGGGCGATAAAACGGCTCAGCGCCTGGTTGGCCTGCTTAACGCAGGCTTCGAGTTGCTGCGGAAAGT > NZ_CP009273/436386‑436553 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |