| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 4,504,618 | T→C | D626G (GAT→GGT) | fecA ← | Fe(3+) dicitrate transport protein FecA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,504,618 | 0 | T | C | 94.1% | 104.8 / ‑0.7 | 34 | D626G (GAT→GGT) | fecA | Fe(3+) dicitrate transport protein FecA |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (12/20); total (13/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.90e-01 | |||||||||||
TTCACATACGCATAGCTGGCGTAGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGATCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACGGTGTCGTTGGTCTGGTTGGAGTCGTACTG > NZ_CP009273/4504555‑4504703 | ttCACATACGCATAGCTGGCGTAGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCg > 1:485596/1‑90 (MQ=255) cGCATAGCTGGCGTAGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGgcg < 1:203841/90‑1 (MQ=255) cATAGCTGGCGTAGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGcgcg > 1:30972/1‑90 (MQ=255) cATAGCTGGCGTAGATGGAAACGTTATAAAGCGTTGTCGTTAGCGTACCCAGACCGTAACGTGCCTTCGTTTCCAGCCCGGTATGGcgcg > 2:496412/1‑90 (MQ=255) tAGCTGGCGTAGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGtt > 2:73601/1‑90 (MQ=255) tAGCTGGCGTAGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGtt < 2:213032/90‑1 (MQ=255) gCTGGCGTAGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGtttt > 1:382243/1‑90 (MQ=255) ggCGTAGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCt > 1:31413/1‑59 (MQ=255) ggCGTAGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCt < 2:31413/59‑1 (MQ=255) gCGTAGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGATCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCa > 2:275513/1‑90 (MQ=255) gCGTAGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCa > 1:306068/1‑90 (MQ=255) gCGTAGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCa < 2:398995/90‑1 (MQ=255) gCGTAGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCa < 2:188813/90‑1 (MQ=255) gCGTAGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGGTTTGCCa > 2:124009/1‑90 (MQ=255) tAGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGt < 2:93042/90‑1 (MQ=255) aGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTg > 2:224273/1‑90 (MQ=255) aTGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCa > 2:419935/1‑90 (MQ=255) aaCGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGAc < 2:18923/90‑1 (MQ=255) aCGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACg < 1:335497/90‑1 (MQ=255) cGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACgg < 1:182777/90‑1 (MQ=255) cGTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACgg < 2:279483/90‑1 (MQ=255) gTTATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACGgt < 2:19269/90‑1 (MQ=255) ttATCAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACGgtg > 1:433805/1‑90 (MQ=255) cAAGCGTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACGGTGTCGt < 2:176518/90‑1 (MQ=255) gTTGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACGGTGTCGTTGGTc < 2:485596/90‑1 (MQ=255) ttGGCGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACGGTGTCGTTGGTCt < 2:30972/90‑1 (MQ=255) cGTTAGCGTACCCAGATCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACGGTGTCGTTGGTCTGGtt < 1:275513/90‑1 (MQ=255) cGTTAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACGGTGTCGTTGGTCTGGtt < 2:306068/90‑1 (MQ=255) ttAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACGGTGTCGTTGGTCTGGTTgg < 1:419935/90‑1 (MQ=255) ttAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACGGTGTCGTTGGTCTGGTTgg < 2:495811/90‑1 (MQ=255) ttAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACGGTGTCGTTGGTCTGGTTgg < 1:76995/90‑1 (MQ=255) tAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACGGTGTCGTTGGTc > 2:108221/1‑82 (MQ=255) tAGCGTACCCAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACGGTGTCGTTGGTc < 1:108221/82‑1 (MQ=255) cAGACCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACGGTGTCGTTGGTCTGGTTGGAGTCGTACTg < 2:102201/90‑1 (MQ=255) | TTCACATACGCATAGCTGGCGTAGATGGAAACGTTATCAAGCGTTGGCGTTAGCGTACCCAGATCGTAACGTGCCTGCGTTTCCAGCCCGGTATGGCGCGTTTTGCCACGTGCAGTGACGGTGTCGTTGGTCTGGTTGGAGTCGTACTG > NZ_CP009273/4504555‑4504703 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |