Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,590,291	T→C	I168V (ATC→GTC)	dnaC ←	DNA replication protein DnaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,590,291	0	T	C	100.0%	102.6 / NA	29	I168V (ATC→GTC)	dnaC	DNA replication protein DnaC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/21);  total (8/21)

TTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGATCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCC  >  NZ_CP009273/4590209‑4590373                                                                                   |                                                                                   ttGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACcagc                                                                             <  2:277281/90‑1 (MQ=255) ttGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACcagc                                                                             <  1:300402/90‑1 (MQ=255)      agaagaGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACCAGCAGaac                                                                        <  2:84830/90‑3 (MQ=255)         agagCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACCAGCAGATCAAc                                                                     >  1:299260/1‑90 (MQ=255)          gagCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACCAGCAGATCAACg                                                                    >  1:173345/1‑90 (MQ=255)           agCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACCAGCAGATCAACGt                                                                   <  1:158467/90‑1 (MQ=255)           agCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACCAGCAGATCAACGt                                                                   <  1:511113/90‑1 (MQ=255)              ggcgATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACCAGCAGATCAACGTTGc                                                                >  2:408686/1‑90 (MQ=255)                 gATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACCAGCAGATCAACGTTGCTCa                                                             <  1:521801/90‑1 (MQ=255)                          tCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTaa                                                    <  1:294294/90‑1 (MQ=255)                          tCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTaa                                                    <  1:408686/90‑1 (MQ=255)                                   tgatCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAgt                                               <  1:478169/86‑1 (MQ=255)                                   tgatCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAgt                                               >  2:478169/1‑86 (MQ=255)                                     atCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACCAGCAGATc                                                                        >  1:479828/1‑59 (MQ=255)                                     atCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACCAGCAGATc                                                                        <  2:479828/59‑1 (MQ=255)                                     atCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGttct                                         <  1:338510/90‑1 (MQ=255)                                       cACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGttcttc                                       >  1:198663/1‑90 (MQ=255)                                                     tCGATTCGGTCTGCACGCCGATCTCATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTAt                         <  2:395443/90‑1 (MQ=255)                                                                     gCCGATCTCATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCttt         >  2:152348/1‑90 (MQ=255)                                                                      ccGATCTCATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTc        <  1:244933/90‑1 (MQ=255)                                                                       cGATCTCATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCa       >  1:257662/1‑90 (MQ=255)                                                                            tcATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGcc  <  2:386181/90‑1 (MQ=255)                                                                            tcATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGcc  <  2:299260/90‑1 (MQ=255)                                                                            tcATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGcc  <  2:257662/90‑1 (MQ=255)                                                                            tcATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGcc  <  2:198663/90‑1 (MQ=255)                                                                            tcATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGcc  <  2:193787/90‑1 (MQ=255)                                                                            tcATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGcc  <  2:485094/90‑1 (MQ=255)                                                                            tcATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGcc  <  2:70942/90‑1 (MQ=255)                                                                            tcATCGACCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGcc  <  1:442129/90‑1 (MQ=255)                                                                                   |                                                                                   TTGGAAGAAGAGCGGCGATCGACGATCTGGTTGATGATCACTTTTTCGTATTTCGATTCGGTCTGCACGCCGATCTCATCGATCACCAGCAGATCAACGTTGCTCAGATCGTTAAGCAGTTGTTCTTCGCTGGTACCGCTATTCCTGAAGGTATCTTTCATCGCC  >  NZ_CP009273/4590209‑4590373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑