Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,595,668	A→G	G16G (GGA→GGG)	yjjZ →	DUF1435 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,595,668	0	A	G	100.0%	101.2 / NA	28	G16G (GGA→GGG)	yjjZ	DUF1435 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/10);  total (18/10)

ATGATATTGGTTATCATTATCAATGAAAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGATTAGTATTGACGCCGAT  >  NZ_CP009273/4595582‑4595754                                                                                       |                                                                                       atGATATTGGTTATCATTATCAATGAAAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGGTTg                                                                                     >  2:41750/1‑90 (MQ=255)    atatTGGTTATCATTATCAATGAAAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTg                                                                                  <  1:38019/90‑1 (MQ=255)             atcattatcaATGAAAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAggg                                                                         >  1:300225/1‑90 (MQ=255)              tcattatcaATGAAAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGc                                                                        >  1:282350/1‑90 (MQ=255)                   atcaATGAAAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATg                                                                   >  2:355816/1‑90 (MQ=255)                                       cATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGGGTTGCTGCCGGGGTCGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGa                                               >  1:431613/1‑90 (MQ=255)                                         tGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGGTGTATGCGGATTTATCGTCAGATc                                             >  2:279609/1‑90 (MQ=255)                                             gCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTg                                         <  1:260993/90‑1 (MQ=255)                                               aaCGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGc                                       >  2:238002/1‑90 (MQ=255)                                               aaCGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGc                                       >  2:387336/1‑90 (MQ=255)                                                  gTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGa                                    >  2:104308/1‑90 (MQ=255)                                                   tACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGAt                                   <  1:387336/90‑1 (MQ=255)                                                   tACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGAt                                   <  1:238002/90‑1 (MQ=255)                                                    aCGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGAtt                                  >  1:135004/1‑90 (MQ=255)                                                      gCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGt                                <  2:300225/90‑1 (MQ=255)                                                         gggCAGTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCAt                             >  2:459720/1‑90 (MQ=255)                                                         gggCAGTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCAt                             >  2:46982/1‑90 (MQ=255)                                                         gggCAGTGGCTGGGGAGTGTTGCTGCCGGGGGTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGAATGGCAt                             >  2:237843/1‑90 (MQ=255)                                                            cAGTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCt                          >  1:377251/1‑90 (MQ=255)                                                              gTGGCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGa                        >  1:158278/1‑90 (MQ=255)                                                                ggCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGATg                      <  2:142972/90‑1 (MQ=255)                                                                ggCTGGGGAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGATg                      <  1:459720/90‑1 (MQ=255)                                                                     gggAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGAtt                 <  2:325636/90‑1 (MQ=255)                                                                      ggAGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGATTa                <  2:331409/90‑1 (MQ=255)                                                                        aGTGTTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGATTAGt              >  1:338849/1‑90 (MQ=255)                                                                           gtTGCTGCCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGATTAGTAtt           >  1:454340/1‑90 (MQ=255)                                                                              gctgcCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCgg                                     <  2:203699/61‑1 (MQ=255)                                                                              gctgcCGGGGTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCgg                                     >  1:203699/1‑61 (MQ=255)                                                                                    ggggTTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGATTAGTATTGACGCCgat  <  2:509863/90‑1 (MQ=255)                                                                                       |                                                                                       ATGATATTGGTTATCATTATCAATGAAAGAGATGAAATCATGTTGCAACGTACGCTGGGCAGTGGCTGGGGAGTGTTGCTGCCGGGATTGCTGATTGCAGGGCTGATGTATGCGGATTTATCGTCAGATCAGTGGCGGATTGTCATTCTGATGGGATTAGTATTGACGCCGAT  >  NZ_CP009273/4595582‑4595754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑