Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,616,816	T→C	V363A (GTC→GCC)	radA →	DNA repair protein RadA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,616,816	0	T	C	100.0%	105.5 / NA	30	V363A (GTC→GCC)	radA	DNA repair protein RadA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/15);  total (15/15)

AAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGC  >  NZ_CP009273/4616733‑4616902                                                                                    |                                                                                       aaaCCGTCTGGCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGCcggcgg                                                                                  >  2:344459/1‑90 (MQ=255) aaaCCGTCTGGCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGCcggcgg                                                                                  >  1:462507/1‑90 (MQ=255)     cGTCTGGCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGCCGGCGGCGTg                                                                              <  2:400314/90‑1 (MQ=255)          ggCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGt                                                                         <  1:240839/90‑1 (MQ=255)          ggCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGt                                                                         <  2:275032/90‑1 (MQ=255)                    ctggctgTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAAcc                                                               >  2:70417/1‑90 (MQ=255)                               gCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACtt                                                    <  1:344459/90‑1 (MQ=255)                                cACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTa                                                   <  2:9541/90‑1 (MQ=255)                                cACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTa                                                   <  1:363176/90‑1 (MQ=255)                                    gTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGCcggc                                                                                    <  2:468857/53‑1 (MQ=255)                                    gTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGCcggc                                                                                    >  1:468857/1‑53 (MQ=255)                                                tGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGa                                   <  1:99111/90‑1 (MQ=255)                                                  cAAATGGCCGATCAGGATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATg                                 <  2:237221/90‑1 (MQ=255)                                                      tGGCCGATCAGGATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGttt                             >  1:200301/1‑90 (MQ=255)                                                      tGGCCGATCAGGATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGttt                             >  1:424410/1‑90 (MQ=255)                                                       ggCCGATCAGGATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTc                            <  2:424410/90‑1 (MQ=255)                                                       ggCCGATCAGGATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTc                            >  1:114290/1‑90 (MQ=255)                                                         ccGATCAGGATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCa                          >  2:453723/1‑90 (MQ=255)                                                           gATCAGGATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGc                        >  1:52573/1‑90 (MQ=255)                                                                 gATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGtt                                              >  1:369949/1‑62 (MQ=255)                                                                 gATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGtt                                              <  2:369949/62‑1 (MQ=255)                                                                 gATGTGTTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTgcgc                  <  2:200301/90‑1 (MQ=255)                                                                   tgtgTTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGa                >  2:433204/1‑90 (MQ=255)                                                                     tgtTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACa              >  1:318062/1‑90 (MQ=255)                                                                      gtTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCg                                                                    <  2:274172/35‑1 (MQ=38)                                                                      gtTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCg                                                                    >  1:274172/1‑35 (MQ=38)                                                                      gtTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAAcc                                                               <  2:319431/40‑1 (MQ=39)                                                                      gtTTGTGAACGTGGCCGGCGGCGTGAAGGTAACCGAAAcc                                                               >  1:319431/1‑40 (MQ=39)                                                                              aCGTGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGc     >  2:183917/1‑90 (MQ=255)                                                                                 tGGCCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGc  <  2:446521/90‑1 (MQ=255)                                                                                    |                                                                                       AAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGC  >  NZ_CP009273/4616733‑4616902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑