Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	471,023	G→A	P63P (CCG→CCA)	ybaY →	YbaY family lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	471,023	0	G	A	100.0%	52.3 / NA	16	P63P (CCG→CCA)	ybaY	YbaY family lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/8);  total (8/8)

ACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGC  >  NZ_CP009273/470937‑471105                                                                                       |                                                                                   aCGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCACCt                                                                                 <  2:151530/90‑1 (MQ=255)     cTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCACCTGAtg                                                                             >  1:500777/1‑90 (MQ=255)               aacacaacaACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCACCTGATGCTGTGCTGAc                                                                   >  1:282663/1‑90 (MQ=255)                           aGCTATCCAGCAACCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCACCTGATGCTGTGCTGACCGTGACACTTTc                                                       >  1:10533/1‑90 (MQ=255)                                                 tcCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCACCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCa                                 <  1:238220/90‑1 (MQ=255)                                                 tcCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCACCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCa                                 <  2:254601/90‑1 (MQ=255)                                                 tcCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCACCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCa                                 <  2:379098/90‑1 (MQ=255)                                                  ccGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCACCTGAtgc                                                                            >  1:299927/1‑46 (MQ=255)                                                  ccGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCACCTGAtgc                                                                            <  2:299927/46‑1 (MQ=255)                                                   cGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCACCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCAcc                               <  2:500777/90‑1 (MQ=255)                                                        ccGTCTGGATCCGTCAGAAAGTCGCACTGCCACCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCaa                          >  1:382920/1‑90 (MQ=255)                                                          gTCTGGATCCGTCAGAAAGTCGCACTGCCACCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCaaaa                        >  2:288984/1‑90 (MQ=255)                                                          gTCTGGATCCGTCAGAAAGTCGCACTGCCACCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCaaaa                        >  2:69657/1‑90 (MQ=255)                                                               gATCCGTCAGAAAGTCGCACTGCCACCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCt                   <  1:442495/90‑1 (MQ=255)                                                                    gTCAGAAAGTCGCACTGCCACCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGgcgc              >  1:85569/1‑90 (MQ=255)                                                                                cACTGCCACCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGc  <  1:377160/90‑1 (MQ=255)                                                                                       |                                                                                   ACGTCTATTTCAGCAACACAACAACCAGCTATCCAGCAACCGAATGTCTCCGGTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGC  >  NZ_CP009273/470937‑471105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑