Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	497,636	T→C	G353G (GGA→GGG)	ybaL ←	Kef family K(+) transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	497,636	0	T	C	100.0%	68.6 / NA	20	G353G (GGA→GGG)	ybaL	Kef family K(+) transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/10);  total (10/10)

GGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTT  >  NZ_CP009273/497553‑497703                                                                                    |                                                                    gggTTGACCAAAATCGACAGGACCCCCCCTGCCAGTACCAGGTTTTGCCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGcc                                                               <  2:456085/90‑1 (MQ=255)      gAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGAt                                                          >  1:136454/1‑90 (MQ=255)      gAGCAAAACCAACAGAACCCCCCCTCCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGAt                                                          <  2:296443/90‑1 (MQ=255)                       tCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCaa                                         >  1:460376/1‑90 (MQ=255)                         gCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATc                                       >  2:341037/1‑90 (MQ=255)                              tGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATCTgcgc                                  >  2:484397/1‑90 (MQ=255)                                   gTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAAc                                                <  1:392073/71‑1 (MQ=255)                                   gTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAAc                                                >  2:392073/1‑71 (MQ=255)                                    tACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCt                            >  2:486451/1‑90 (MQ=255)                                     aCCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTg                           <  1:496488/90‑1 (MQ=255)                                       cAGGTTTTGTCGGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTggc                         <  2:268579/90‑1 (MQ=255)                                        aGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTggcg                        >  1:408668/1‑90 (MQ=255)                                            tttGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGa                    <  2:93179/90‑1 (MQ=255)                                               gTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATgg                 >  2:162479/1‑90 (MQ=255)                                               gTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATgg                 >  1:287686/1‑90 (MQ=255)                                                   ggCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTaa             <  1:248145/90‑1 (MQ=255)                                                    gCCTGCGGCAGTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAAt            >  1:243396/1‑90 (MQ=255)                                                         cGGCAGTAAATTCAATGCCATCCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGt       <  2:493119/90‑1 (MQ=255)                                                         cGGCAGTAAATTCAATCCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGt       <  2:332526/90‑1 (MQ=255)                                                              gTAAATTCAATGCCATTCCCAGCCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGtt  <  1:280697/90‑1 (MQ=255)                                                                                    |                                                                    GGGTTGAGCATAATCGACAGGATCGCCCCTGCCAGTACCAGGTTTTGTCCGGCCTGCGGCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGATGGTTAATGCCGTACGTT  >  NZ_CP009273/497553‑497703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑