Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	527,001	T→C	E226G (GAA→GGA)	allS ←	HTH‑type transcriptional activator AllS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	527,001	0	T	C	100.0%	34.6 / NA	11	E226G (GAA→GGA)	allS	HTH‑type transcriptional activator AllS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/8);  total (3/8)

GTTGTTGATTATCGATCATTGACTGGCAAAGCGATTTTGGCAAAAAACCAATGCCAACGCCCGCCAGATGGGCGGCGATTTTCGTTTCCATATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTC  >  NZ_CP009273/526915‑527080                                                                                       |                                                                                gttgttGATTATCGATCATTGACTGGCAAAGCGATTTTGGCAAAAAACCAATGCCAACGCCCGCCAGATGGGCGGCGATTTTCGTTCCCa                                                                              <  2:12055/90‑1 (MQ=255)         ttATCGATCATTGACTGGCAAAGCGATTTTGGCAAAAAACCAATGCCAACGCCCGCCAGATGGGCGGCGATTTTCGTTCCCATATCGGGa                                                                      <  2:172493/90‑1 (MQ=255)           aTCGATCATTGACTGGCAAAGCGATTTTGGCAAAAAACCAATGCCAACGCCCGCCAGATGGGCGGCGATTTTCGTTCCCATATCGGGAAc                                                                    >  1:488212/1‑90 (MQ=255)                         ggCAAAGCGATTTTGGCAAAAAACCAATGCCAACGCCCGCCAGATGGGCGGCGATTTTCGTTCCCATATCGGGAACAATAATCTCttttt                                                      >  1:119486/1‑90 (MQ=255)                                                 cAATGCCAACGCCCGCCAGATGGGCGGCGATTTTCGTTCCCATATCg                                                                         >  1:389863/1‑47 (MQ=255)                                                 cAATGCCAACGCCCGCCAGATGGGCGGCGATTTTCGTTCCCATATCg                                                                         <  2:389863/47‑1 (MQ=255)                                                   aTGCCAACGCCCGCCAGATGGGCGGCGATTTTCGTTCCCATATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGt                            <  2:119486/90‑1 (MQ=255)                                                           gcccgccAGATGGGCGGCGATTTTCGTTCCCATATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTaa                    <  1:63220/90‑1 (MQ=255)                                                                        gcggcgATTTTCGTTCCCATATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTg       <  2:258153/90‑1 (MQ=255)                                                                        gcggcgATTTTCGTTCCCATATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTg       <  2:280992/90‑1 (MQ=255)                                                                             gATTTTCGTTCCCATATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTc  <  2:218839/90‑1 (MQ=255)                                                                                       |                                                                                GTTGTTGATTATCGATCATTGACTGGCAAAGCGATTTTGGCAAAAAACCAATGCCAACGCCCGCCAGATGGGCGGCGATTTTCGTTTCCATATCGGGAACAATAATCTCTTTTTGCCCTGGCAATCGCCAGGCGACGCGTTTGGTTAAGGTGCGGGCGCTGTCTTC  >  NZ_CP009273/526915‑527080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑