| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 116,817 | T→C | I408V (ATC→GTC) | aroP ← | aromatic amino acid transporter AroP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 116,817 | 0 | T | C | 95.0% | 66.6 / NA | 20 | I408V (ATC→GTC) | aroP | aromatic amino acid transporter AroP |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (10/9); minor base A (1/0); total (11/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGC > NZ_CP009273/116738‑116901 | gggATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCACGCAGAACCACTTAccc > 2:186053/1‑90 (MQ=255) gATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGACCCAGTTACCCAg < 1:92964/90‑1 (MQ=255) aCCAAAATCGCCTTTCCGGGGGTCATCAGCATAATCCCCAGTACCGCCGCCATAAACAGCAGGCAGACCCAGTTACCCAGCGGATAAagc < 2:214188/90‑1 (MQ=255) cGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGACCCAGTTACCCAGCGGATAAagcag < 2:102792/90‑1 (MQ=255) aaTCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGACCCAGTTACCCAGCGGATAAagcagagc > 1:70063/1‑90 (MQ=255) aTTCCTGGGGTCATCAGCATAATCACCAGTCCCGCCGCCATAAACAGCAGGCAGACCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAg < 2:99401/90‑1 (MQ=255) gggggCCACCAGAAAAACCCCCATTCCCGCCGCCATAAACAGCAGGCAGACCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGt < 2:254116/89‑1 (MQ=255) catcaGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGACCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCAc > 1:51041/1‑90 (MQ=255) catcaGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGACCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCAc > 1:244260/1‑90 (MQ=255) catcaGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGACCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCAc > 2:228003/1‑90 (MQ=255) catcaGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGACCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCAc > 1:196109/1‑90 (MQ=255) aGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGACCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCt < 1:228003/90‑1 (MQ=255) cAGTACCGCCGCCATAAACAGCAGGCAGACCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCtt > 1:358116/1‑90 (MQ=255) cAGTACCGCCGCCATAAACAGCAGGCAGACCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCtt > 1:302816/1‑90 (MQ=255) gccgccATAAACAGCAGGCAGACCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGa > 2:71074/1‑90 (MQ=255) cgccATAAACAGCAGGCAGACCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACg > 1:214847/1‑90 (MQ=255) agcagGCAGACCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCata < 1:429349/90‑1 (MQ=255) gcaggcagACCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCatat < 2:338592/90‑1 (MQ=255) gcaggcagACCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCatat > 2:382299/1‑90 (MQ=255) gcagACCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATgcgc < 1:381510/90‑1 (MQ=255) | GGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGC > NZ_CP009273/116738‑116901 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |