Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	594,745	T→C	D192D (GAT→GAC)	cusA →	Cu(+)/Ag(+) efflux RND transporter permease subunit CusA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	594,745	0	T	C	100.0%	32.0 / NA	10	D192D (GAT→GAC)	cusA	Cu(+)/Ag(+) efflux RND transporter permease subunit CusA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/7);  total (3/7)

AAATATGAGCTGAAAACCATCCCTGACGTTGCGGAAGTGGCGTCGGTGGGCGGTGTGGTGAAAGAGTATCAGGTGGTTATCGATCCCCAGCGCCTGGCGCAGTATGGCATCAGTCTCGCCGAAGTAAAAAGCGCGCTGGATGCTTCAAACCAGGAAGCGGGCGGTT  >  NZ_CP009273/594662‑594827                                                                                    |                                                                                   aaaTATGAGCTGAAAACCATCCCTGACGTTGCGGAAGTGGCGTCGGTGGGCGGTGTGGTGAAAGAGTATCAGGTGGTTATCGACCCCCAg                                                                              <  1:62456/90‑1 (MQ=255)      tGAGCTGAAAACCATCCCTGACGTTGCGGAAGTGGCGTCGGTGGGCGGTGTGGTGAAAGAGTATCAGGTGGTTATCGACCCCCAGCGCCt                                                                         <  2:271984/90‑1 (MQ=255)      tGAGCTGAAAACCATCCCTGACGTTGCGGAAGTGGCGTCGGTGGGCGGTGTGGTGAAAGAGTATCAGGTGGTTATCGACCCCCAGCGCCt                                                                         <  2:503073/90‑1 (MQ=255)           tGAAAACCATCCCTGACGTTGCGGAAGTGGCGTCGGTGGGCGGTGTGGTGAAAGAGTATCAGGTGGTTATCGACCCCCAGCGCCTGgcgc                                                                    <  1:155090/90‑1 (MQ=255)           tGAAAACCATCCCTGACGTTGCGGAAGTGGCGTCGGTGGGCGGTGTGGTGAAAGAGTATCAGGTGGTTATCGACCCCCAGCGCCTGgcgc                                                                    <  2:522543/90‑1 (MQ=255)            gAAAACCATCCCTGACGTTGCGGAAGTGGCGTCGGTGGGCGGTGTGGTGAAAGAGTATCAGGTGGTTATCGACCCCCAGCGCCTGGCGCa                                                                   <  2:59082/90‑1 (MQ=255)                                                        tggtgAAAGAGTATCAGGTGGTTATCGACCCCCAGCGCCTGgcgc                                                                    <  1:355092/45‑1 (MQ=255)                                                        tggtgAAAGAGTATCAGGTGGTTATCGACCCCCAGCGCCTGgcgc                                                                    >  2:355092/1‑45 (MQ=255)                                                                   tATCAGGTGGTTATCGACCCCCAGCGCCTGGCGCAGTATGGCATCAGTCTCGCCGAAGTAAAAAGCGCGCTGGATGCTTCAAACCAGGaa            >  1:239317/1‑90 (MQ=255)                                                                             ttATCGACCCCCAGCGCCTGGCGCAGTATGGCATCAGTCTCGCCGAAGTAAAAAGCGCGCTGGATGCTTCAAACCAGGAAGCGGGCGGtt  >  2:220966/1‑90 (MQ=255)                                                                                    |                                                                                   AAATATGAGCTGAAAACCATCCCTGACGTTGCGGAAGTGGCGTCGGTGGGCGGTGTGGTGAAAGAGTATCAGGTGGTTATCGATCCCCAGCGCCTGGCGCAGTATGGCATCAGTCTCGCCGAAGTAAAAAGCGCGCTGGATGCTTCAAACCAGGAAGCGGGCGGTT  >  NZ_CP009273/594662‑594827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑