Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	643,749	T→C	T427A (ACC→GCC)	citF ←	citrate lyase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	643,749	0	T	C	100.0%	73.9 / NA	21	T427A (ACC→GCC)	citF	citrate lyase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/12);  total (9/12)

TGCCAGTTCCGGACGTGCCGGGTTAACTGCGATACCGTGGTCTGTGACCAGAATATCGACACTGGAGCCTGGGGTGATGCAGGTCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTA  >  NZ_CP009273/643666‑643831                                                                                    |                                                                                   tGCCAGTTCCGGACGTGCCGGGTTAACTGCGATACCGTGGTCTGTGACCAGAATATCGACACTGGAGCCTGGGGTGATGCAGGCCAGTAc                                                                              <  2:10580/90‑1 (MQ=255)                 gCCGGGTTAACTGCGATACCGTGGTCTGTGACCAGAATATCGACACTGGAGCCTGGGGTGATGCAGGCCAGTACGTTATCCACCAGAGTc                                                              <  2:343883/90‑1 (MQ=255)                        tAACTGCGATACCGTGGTCTGTGACCAGAATATCGACACTGGAGCCTGGGGTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATAc                                                       <  1:60597/90‑1 (MQ=255)                                     gTGGTCTGTGACCAGAATATCGACACTGGAGCCTGGGGTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAg                                          >  2:501430/1‑90 (MQ=255)                                     gTGGTCTGTGACCAGAATATCGACACTGGAGCCTGGGGTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAg                                          >  2:383643/1‑90 (MQ=255)                                     gTGGTCTGTGACCAGAATATCGACACTGGAGCCTGGGGTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAg                                          >  1:10149/1‑90 (MQ=255)                                      tGGTCTGTGACCAGAATATCGACACTGGAGCCTGGGGTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAgc                                         <  1:383643/90‑1 (MQ=255)                                          cTGTGACCAGAATATCGACACTGGAGCCTGGGGTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACcgc                                                 <  2:515060/78‑1 (MQ=255)                                          cTGTGACCAGAATATCGACACTGGAGCCTGGGGTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACcgc                                                 >  1:515060/1‑78 (MQ=255)                                              gACCAGAATATCGACACTGGAGCCTGGGGTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGcgac                                 <  1:501430/90‑1 (MQ=255)                                                             acTGGAGCCTGGGGTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAgcgc                  >  1:201290/1‑90 (MQ=255)                                                                ggAGCCTGGGGTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCaga               >  2:316183/1‑90 (MQ=255)                                                                  aGCCTGGGGTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAgg             <  1:139233/90‑1 (MQ=255)                                                                    ccTGGGGTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCa           <  2:328596/90‑1 (MQ=255)                                                                     cTGGGGTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCaa          >  2:378074/1‑90 (MQ=255)                                                                         ggTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATcgc      <  1:452471/90‑1 (MQ=255)                                                                         ggTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATcgc      <  1:392879/90‑1 (MQ=255)                                                                         ggTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATcgc      <  1:316183/90‑1 (MQ=255)                                                                         ggTGATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATcgc      <  1:201151/90‑1 (MQ=255)                                                                            gATGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGt   >  1:231524/1‑90 (MQ=255)                                                                             aTGCAGGCCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTa  >  1:457347/1‑90 (MQ=255)                                                                                    |                                                                                   TGCCAGTTCCGGACGTGCCGGGTTAACTGCGATACCGTGGTCTGTGACCAGAATATCGACACTGGAGCCTGGGGTGATGCAGGTCAGTACGTTATCCACCAGAGTCGGAATACGACCGCGTACCAGCGGCGCGACGATGATGGAAAGCGCAGAGGCAATCGCGGTA  >  NZ_CP009273/643666‑643831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑