Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	683,001	T→C	D68G (GAT→GGT)	gltI ←	glutamate/aspartate ABC transporter substrate‑binding protein GltI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	683,001	0	T	C	100.0%	16.3 / NA	6	D68G (GAT→GGT)	gltI	glutamate/aspartate ABC transporter substrate‑binding protein GltI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/4);  total (2/4)

AGGTAATCGGAATCAGTTTTACCTGCAAGTCCGGTTTGTTGAGTTTCTTTTTCACTGCTTCAACAATGGCGTTGGAGTAATCCTGCGAGTAACCCACCACTTTTTGCTGATTGTCGTAATAAGAGAAAGGCACTGAAGATTCACGGTGACCGACGACA  >  NZ_CP009273/682921‑683078                                                                                 |                                                                              aGGTAATCGGAATCAGTTTTACCTGCAAGTCCGGTTTGTTGAGTTTCTTTTTCACTGCTTCAACAATGGCGTTGGAGTAACCCTGCGAGt                                                                      <  2:444671/90‑1 (MQ=255)   gTAATCGGAATCAGTTTTACCTGCAAGTCCGGTTTGTTGAGTTTCTTTTTCACTGCTTCAACAATGGCGTTGGAGTAACCCTGCGAGTaa                                                                    >  2:251592/1‑90 (MQ=255)                                        tgAGTTTCTTTTTCACTGCTTCAACAATGGCGTTGGAGTAACCCTGCGAGTAACCCACCACTTTTTGCTGATTGTCGTAATAAGAGAAAg                               <  1:135300/90‑1 (MQ=255)                                             ttCTTTTTCACTGCTTCAACAATGGCGTTGGAGTAACCCTGCGAGTAACCCACCACTTTTTGCTGATTGTCGTAATAAGAGAAAGGCACt                          <  2:125595/90‑1 (MQ=255)                                                         gCTTCAACAATGGCGTTGGAGTAACCCTGCGAGTAACCCACCACTTTTTGCTGATTGTCGTAATAAGAGAAAGGCACTGAAGATTCACgg              <  2:323700/90‑1 (MQ=255)                                                                     gCGTTGGAGTAACCCTGCGAGTAACCCACCACTTTTTGCTGATTGTCGTAATAAGAGAAAGGCACTGAAGATTCACGGTGACCGACGACa  >  2:75541/1‑90 (MQ=255)                                                                                 |                                                                              AGGTAATCGGAATCAGTTTTACCTGCAAGTCCGGTTTGTTGAGTTTCTTTTTCACTGCTTCAACAATGGCGTTGGAGTAATCCTGCGAGTAACCCACCACTTTTTGCTGATTGTCGTAATAAGAGAAAGGCACTGAAGATTCACGGTGACCGACGACA  >  NZ_CP009273/682921‑683078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑