| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 762,108 | C→T | G203G (GGC→GGT) | mngA → | PTS 2‑O‑a‑mannosyl‑D‑glycerate transporter subunit IIABC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 762,108 | 0 | C | T | 87.5% | 35.4 / ‑6.4 | 17 | G203G (GGC→GGT) | mngA | PTS 2‑O‑a‑mannosyl‑D‑glycerate transporter subunit IIABC |
| Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (8/6); minor base G (1/1); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCA > NZ_CP009273/762024‑762187 | gaTTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGGGCTaa > 2:335002/2‑90 (MQ=255) atatGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGTGCTAACGGCATTGAAGGGc > 2:230363/1‑90 (MQ=255) gCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGTGCTAACGGCATTGAAGGGc < 2:395983/85‑1 (MQ=255) gCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGTGCTAACGGCATTGAAGGGc > 1:395983/1‑85 (MQ=255) gCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGTGCTAACGGCATTGAAGGGCGTTTa > 1:150865/1‑90 (MQ=255) gCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGTGCTAACGGCATTGAAGGGCGTTTa > 1:350101/1‑90 (MQ=255) gCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTa > 2:330007/1‑90 (MQ=255) tGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGTGCTAACGGCATTGAAggg < 2:476385/82‑1 (MQ=255) tGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGTGCTAACGGCATTGAAggg > 1:476385/1‑82 (MQ=255) tGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGTGCTAACGGCATTGAAGGGCGTTTAAc > 1:153095/1‑90 (MQ=255) gCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGTGCTAACGGCATTGAAGGGCGTTTAAcg > 1:521776/1‑90 (MQ=255) atatCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGTGCTAACGGCATTGAAGGGCGTTTAACGGCGGa < 2:213/90‑1 (MQ=255) aaaaaGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGTGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCa < 2:206502/90‑1 (MQ=255) tttaaaaacCAGGGGGCAAAGGGCATGGAGGGGCGTTTACCGGCGGATCAACTAAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTc < 1:247166/86‑1 (MQ=255) taaaaaaCAGGGTGCTACCGGCATTGAGGGGCTTTAAACGGCGGATCAACTCAATAGTGAAACCGCCTGTATTTTTGCGGCTGAAGTCGc < 1:387762/88‑1 (MQ=255) tGAAAAACAAGGTGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCCCCTGTATTTTTTCGGGTGAAggctc > 1:491014/1‑86 (MQ=255) aaaaaaaaGGTGCAACCGGCATTGAGGGGCGTTTAACGGCGAATCAACTAAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCa < 1:32077/90‑1 (MQ=255) | TATTGCTCACACCTATATGGCTGCGGAATATCTGGAAAAAGCCGGACGCAAACTCGGCGTAAATGTTTACGTTGAAAAACAAGGCGCTAACGGCATTGAAGGGCGTTTAACGGCGGATCAACTCAATAGTGCAACCGCCTGTATTTTTGCGGCTGAAGTCGCCA > NZ_CP009273/762024‑762187 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |