| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 778,272 | T→C | S244S (AGT→AGC) | nadA → | quinolinate synthase NadA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 778,272 | 0 | T | C | 100.0% | 13.4 / NA | 6 | S244S (AGT→AGC) | nadA | quinolinate synthase NadA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (3/3); total (3/3) | |||||||||||
GGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGTCAACTGATCGCTGCTGCGAAAACATTGCCACATCAGAGGCTTATTGTGGCAACCGATCGGGG > NZ_CP009273/778197‑778334 | ggATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGGCGGGTCCACCCGCCAACTGATcgccgc > 1:372459/1‑90 (MQ=255) ggTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGCCAACTGATCGCTGCTGCGAAAACATTGcc < 1:20340/90‑1 (MQ=255) ttGTCGATATGGCGGATGCGGGCGGTTCCACCAGCCAACTGATCGCTGCTGCGAAAACATTTCCACATCAGAGGGTTATTTTGGCAACCg > 2:141940/1‑90 (MQ=255) cGATATGGCGGATGCGGTCGGTTCCACCAGCCAACTGATCGCTGCTGCGAAAACATTGCCACATCAGAGGCTTATTGTGGCAACCGATCg < 2:372459/90‑1 (MQ=255) tGCGGTCGGTTCCACCAGCCAACTGATCGCTGCTGCGAAAACATTGCCACATCAGAGGCTTATTGTGGCAACCGATCgggg > 1:30403/1‑81 (MQ=255) tGCGGTCGGTTCCACCAGCCAACTGATCGCTGCTGCGAAAACATTGCCACATCAGAGGCTTATTGTGGCAACCGATCgggg < 2:30403/81‑1 (MQ=255) | GGATGCTGCCATACTGGTGCATCCAGAATCACCACAAGCTATTGTCGATATGGCGGATGCGGTCGGTTCCACCAGTCAACTGATCGCTGCTGCGAAAACATTGCCACATCAGAGGCTTATTGTGGCAACCGATCGGGG > NZ_CP009273/778197‑778334 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |