Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	121,596	A→G	H698R (CAC→CGC)	aceE →	pyruvate dehydrogenase (acetyl‑transferring), homodimeric type

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	121,596	0	A	G	100.0%	101.3 / NA	29	H698R (CAC→CGC)	aceE	pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/15);  total (14/15)

ATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGG  >  NZ_CP009273/121514‑121668                                                                                   |                                                                         atgcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCGCATGCCg                                                                   <  1:380338/90‑1 (MQ=255)   gcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCGCATGCCGGc                                                                 <  2:222222/90‑1 (MQ=255)   gcatgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCGCATGCCGGc                                                                 <  1:395921/90‑1 (MQ=255)     atgACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCGCATGCCGGCaa                                                               >  2:73718/1‑90 (MQ=255)             cTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGaa                                                       >  2:420874/1‑90 (MQ=255)                  gCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGc                                                  >  1:384224/1‑90 (MQ=255)                    gTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTg                                                >  2:212456/1‑90 (MQ=255)                       tGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAgg                                             <  2:418288/90‑1 (MQ=255)                             gTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTa                                       <  2:384224/90‑1 (MQ=255)                              tGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTAt                                      >  2:94422/1‑90 (MQ=255)                              tGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTAt                                      >  1:451269/1‑90 (MQ=255)                                    aCAAGAGAACGTTTCCTACTACATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTaa                                <  1:426901/90‑1 (MQ=255)                                       agagAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAgg                             >  2:399424/1‑90 (MQ=255)                                         agaACGTTTACTACTACATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTa                           <  1:73718/90‑1 (MQ=255)                                            aCGTTTACTACTACATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTaa                                >  1:320850/1‑82 (MQ=255)                                            aCGTTTACTACTACATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTaa                                <  2:320850/82‑1 (MQ=255)                                                ttACTACTACATCACTACGCTGAACGAAAACTACAGCATGCCGGCAATGCCGCAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACaa                    <  2:254171/90‑1 (MQ=255)                                                    tactacATCACTACGCTGAACGAAAACTACCGCATGCCGGCa                                                                >  1:470885/1‑42 (MQ=255)                                                    tactacATCACTACGCTGAACGAAAACTACCGCATGCCGGCa                                                                <  2:470885/42‑1 (MQ=255)                                                       tacATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGaa             <  1:495386/90‑1 (MQ=255)                                                        acATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGaaa            >  2:28423/1‑90 (MQ=255)                                                        acATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGaaa            >  2:62306/1‑90 (MQ=255)                                                         cATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAAc           <  2:156643/90‑1 (MQ=255)                                                         cATCACTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAAc           <  2:451269/90‑1 (MQ=255)                                                             aCTACGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTAtt       >  2:432880/1‑90 (MQ=255)                                                                aCGCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGaa    >  1:44585/1‑90 (MQ=255)                                                                  gCTGAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAgg  <  1:461777/90‑1 (MQ=255)                                                                     gAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTa                                       <  1:392694/50‑1 (MQ=255)                                                                     gAACGAAAACTACCGCATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTa                                       >  2:392694/1‑50 (MQ=255)                                                                                   |                                                                         ATGCATGACGGTCTGGAGCGTATGTACGGTGAAAAACAAGAGAACGTTTACTACTACATCACTACGCTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGG  >  NZ_CP009273/121514‑121668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑