Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	839,475	T→C	I488V (ATC→GTC)	ybiO ←	mechanosensitive channel protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	839,475	0	T	C	100.0%	36.0 / NA	13	I488V (ATC→GTC)	ybiO	mechanosensitive channel protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/7);  total (6/7)

GCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTAGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATAC  >  NZ_CP009273/839388‑839531                                                                                        |                                                         gcgcGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTAGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGACCa                                                        >  2:411324/1‑90 (MQ=255)              cAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTAGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGACCAAACTGGCGAGCAc                                           <  2:147493/90‑1 (MQ=255)                          ggTACGCGTGCGGGCGCTGGGTAGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGACCAAACTGGCGAGCACCGTCCAGCCAAc                               >  1:158929/1‑90 (MQ=255)                               gcgTGCGGGCGCTGGGTAGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGACCAAACTGGCGAGCACCGTCCAGCCAAccgccg                          >  1:286018/1‑90 (MQ=255)                                   gggggCGCTGGGTAGGGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGACCAAACTGGCGAGCACCGTCCAGCCAACCGCCGagaa                      <  1:303007/88‑1 (MQ=255)                                   gCGGGCGCTGGGTAGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGACCAAACTGGCGAGCACCGTCCAGCCAACCGCCGagaa                      >  1:63251/1‑90 (MQ=255)                                   gCGGGCGCTGGGTAGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGACCAAACTGGCGAGCACCGTCCAGCCAACCGCCGagaa                      >  1:92647/1‑90 (MQ=255)                                             gttAGCGGGCGGCGGGGAATAGCGGAACCCACCGGGTTTCCGACCAAATTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATg            <  2:158929/88‑1 (MQ=255)                                             ggTAGCGGGCGGCCATAAATATCCGAACCCAGCCGTTTTTCGACCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATg            <  2:111976/90‑1 (MQ=255)                                                 gcgggcggCCATGAATATCCGAAGCCAGCCGGTTTTCGACCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTg        >  2:494376/1‑90 (MQ=255)                                                  cgggcggCCATGAATATCCGAAGCCAGCCGGTTTTCGACCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGc       <  2:63251/90‑1 (MQ=255)                                                      cggcCATGAATATCCGAAGCCAGCCGGTTTTCGACCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATa   <  1:153822/90‑1 (MQ=255)                                                       ggcCATGAATATCCGAAGCCAGCCGGTTTTCGACCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATAc  <  2:286018/90‑1 (MQ=255)                                                                                        |                                                         GCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTAGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATAC  >  NZ_CP009273/839388‑839531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑