| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 847,270 | A→G | S262P (TCC→CCC) | opgE ← | phosphoethanolamine transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 847,270 | 0 | A | G | 90.0% | 22.3 / ‑2.8 | 10 | S262P (TCC→CCC) | opgE | phosphoethanolamine transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (2/7); total (2/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-01 | |||||||||||
GATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAAGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCG > NZ_CP009273/847183‑847346 | gATTTGCCAGGGTGACAACGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAAGCGATTTCTGCGTGGGGc < 1:175656/90‑1 (MQ=255) gggTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAAGCGATTTCTGCGTGGAGCCACTggcgg < 2:92966/90‑1 (MQ=255) gTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAAGCGATTTCTGCGTGGGGCCACTGGCGGCAATGTAGTc < 2:360113/90‑1 (MQ=255) aCTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAAGCGATTTCTGCGTGGGGCCACTGGCGGCAATGTAGTCAGCAAATATCa < 2:489789/90‑1 (MQ=255) gAGCGTTAAGCCAAGCGATTTCTGCGTGGGGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGt > 1:339510/1‑90 (MQ=255) gCGTTAAGCCAAGCGATTTCTGCGTGGGGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTAt < 2:339510/90‑1 (MQ=255) aaGCGATTTCTGCGTGGGGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTg > 2:228237/1‑90 (MQ=255) gCGATTTCTGCGTGGGGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGAc < 1:220935/90‑1 (MQ=255) gCGATTTCTGCGTGGGGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGAc < 2:425873/90‑1 (MQ=255) gATTTCTGCGTGGGGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCg < 1:151529/90‑1 (MQ=255) | GATTTGCCAGGGTGACAAAGTTATCCTGAAACTGTGGTTTGCCATCGACAACGCGATTGAGCGTTAAGCCAAGCGATTTCTGCGTGGAGCCACTGGCGGCAATGTAGTCAGCAAATATCAAACCGTTAACGCTGCTGGCAAACGGGGTATTGTCCCAGTGACCG > NZ_CP009273/847183‑847346 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |