Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	905,181	T→C	D442G (GAT→GGT)	poxB ←	ubiquinone‑dependent pyruvate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	905,181	0	T	C	100.0%	40.5 / NA	13	D442G (GAT→GGT)	poxB	ubiquinone‑dependent pyruvate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/8);  total (5/8)

ATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATCGCCGCACATGGCGACCACCTGACGTTCTGGCTCTGTCGCCTGCGCACCCAGC  >  NZ_CP009273/905103‑905261                                                                               |                                                                                 aTCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAACCGCCCATCAAc                                                                       <  2:106599/90‑1 (MQ=255) aTCGCCACAAAGCCCAGCACGATGTTGTTAAAGACGACAATTTTCCCTGGCAGTTTCACCTGCACTACTGAGAGGAAACCGCCCATCAAc                                                                       <  1:285654/90‑1 (MQ=255)               cAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAACCGCCCATCAACATGCTAAAACCGcc                                                         <  1:270216/90‑1 (MQ=255)                      cTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAACCGCCCATCAAc                                                                       >  1:4221/1‑69 (MQ=255)                      cTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAACCGCCCATCAAc                                                                       <  2:4221/69‑1 (MQ=255)                       tgttgtTAAAGACGCCAATTTCCACTGCCATTTCAATCGGCACTACTGAGAGGAAACCGCCCATAAACATGTTAAAACCGCCATcgccgc                                                 <  2:409134/90‑1 (MQ=255)                                  acgacAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAACCGCCCATCAACATGCTAAAACCGCCATCGCCGCACATGGCGacc                                      >  2:356024/1‑90 (MQ=255)                                    gacAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAACCGCCCATCAACATGCTAAAACCGCCATCGCCGCACATGGCGaccac                                    >  2:186792/1‑90 (MQ=255)                                     acAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAACCGCCCATCAACATGCTAAAACCGCCATCGCCGCACATGGCGaccacc                                   >  1:349626/1‑90 (MQ=255)                                        aTTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAACCGCCCATCAACATGCTAAAACCGCCATCGCCGCACATGGCGACCACCTGa                                <  2:349626/90‑1 (MQ=255)                                                                      gagaGGAAACCGCCCATCAACATGCTAAAACCGCCATCGCCGCACATGGCGACCACCTGACGTTCTGGCTCTGTCGCCTGCGCACCCAgc  <  1:181433/90‑1 (MQ=255)                                                                        gagGAAACCGCCCATCAACATGCTAAAACCGCCATCGCCGCACATGGCGa                                        >  1:127843/1‑50 (MQ=255)                                                                        gagGAAACCGCCCATCAACATGCTAAAACCGCCATCGCCGCACATGGCGa                                        <  2:127843/50‑1 (MQ=255)                                                                               |                                                                                 ATCGCCACAAAGCCCAGCACGCTGTTGTTAAAGACGACAATTTTCACTGGCAGTTTCATCTGCACTACTGAGAGGAAATCGCCCATCAACATGCTAAAACCGCCATCGCCGCACATGGCGACCACCTGACGTTCTGGCTCTGTCGCCTGCGCACCCAGC  >  NZ_CP009273/905103‑905261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑