| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,080,012 | A→G | Y394C (TAC→TGC) | efeB → | iron uptake transporter deferrochelatase/peroxidase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,080,012 | 0 | A | G | 100.0% | 12.5 / NA | 5 | Y394C (TAC→TGC) | efeB | iron uptake transporter deferrochelatase/peroxidase subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (2/3); total (2/3) | |||||||||||
AGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGCCGGGGGTGAAGGACGCGAACGATTATTTCGGAAGCGCGTTATTGC > NZ_CP009273/1079962‑1080095 | aGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATGCGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGcc > 2:262480/1‑90 (MQ=255) gCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATGCGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGCCgg < 1:262480/90‑1 (MQ=255) aCAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATGCGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGCCGGGGGTGAAGGAcgc < 1:249579/90‑1 (MQ=255) tGGAGGAATGCGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGCCGGGGGGGAAGGGCGCGGACGGTTATTTCGGAAGCGCGTTAt > 2:397551/1‑90 (MQ=255) aggaATGCGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGCCGGGGGTGAAGGACGCGAACGATTATTTCGGAAGCGCGTTATTGc < 2:470948/90‑1 (MQ=255) | AGGCTTCCTGACAGTACAAAAAAGGCTCAATGGCGAAGCGCTGGAGGAATACGTTAAACCTATCGGCGGCGGTTATTTTTTTGCGCTGCCGGGGGTGAAGGACGCGAACGATTATTTCGGAAGCGCGTTATTGC > NZ_CP009273/1079962‑1080095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |