Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,097,924	A→G	V25A (GTA→GCA)	csgE ←	curli production assembly/transport protein CsgE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,097,924	0	A	G	100.0%	26.3 / NA	9	V25A (GTA→GCA)	csgE	curli production assembly/transport protein CsgE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/4);  total (5/4)

CGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTT  >  NZ_CP009273/1097864‑1097993                                                             |                                                                      cGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTGCCTCAACGGCGTGAAGATTCCCTgcggcg                                          >  2:138805/1‑90 (MQ=255)                ccAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTGCCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTgcc                           <  2:6365/90‑1 (MQ=255)                   aTAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTGCCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTgccgcc                        >  2:73635/1‑90 (MQ=255)                     aGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTGCCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCcac                      <  1:261006/90‑1 (MQ=255)                         gAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTGCCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATc                  <  1:382170/90‑1 (MQ=255)                             cAGTATGGTCAGTTAGCAATCCCGGGACTTCTGCCTCAACGGCGTGa                                                         >  1:290911/1‑47 (MQ=255)                             cAGTATGGTCAGTTAGCAATCCCGGGACTTCTGCCTCAACGGCGTGa                                                         <  2:290911/47‑1 (MQ=255)                                tATGGTCAGTTAGCAATCCCGGGACTTCTGCCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTa           >  1:509354/1‑90 (MQ=255)                                         ttAGCAATCCCGGGACTTCTGCCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGtt  >  2:68716/1‑90 (MQ=255)                                                             |                                                                      CGGTAAAAATCATGGCCAATAGATGAAACAGTATGGTCAGTTAGCAATCCCGGGACTTCTACCTCAACGGCGTGAAGATTCCCTGCGGCGAACAGAAATTCTGCCGCCACAATCCAGCGTAAATAACGTT  >  NZ_CP009273/1097864‑1097993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑