Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,138,424	T→C	D467G (GAC→GGC)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,138,424	0	T	C	100.0%	38.2 / NA	12	D467G (GAC→GGC)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/2);  total (10/2)

TTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGT  >  NZ_CP009273/1138342‑1138505                                                                                   |                                                                                  ttcttcCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGCCCTGACg                                                                            >  2:146565/1‑90 (MQ=255)         cTTTACGCACGCGCAGCACGTGGTAGTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGCCCTGACGAGTTTCaa                                                                    <  1:229336/90‑1 (MQ=255)           ttACGCACGCGCAGCACGTGGTAGTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGCCCTGACGAGTTTCAATg                                                                  >  2:269840/1‑90 (MQ=255)                              ggTAGTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGCCCTGACGAGTTTCAATGGCATTTACCGCAGAACGtt                                               >  2:248143/1‑90 (MQ=255)                                  gTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGCCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTttc                                           >  1:446292/1‑90 (MQ=255)                                           ttCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGCCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTcagcag                                  >  1:366630/1‑90 (MQ=255)                                              cATCTGATCGTTTGGCACAATTACACAGCGCACACCGCCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTa                               >  2:14890/1‑90 (MQ=255)                                                              acaATTACACAGCGCACACCGCCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGcaca               <  2:366630/90‑1 (MQ=255)                                                                           gcACACCGCCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGt  >  1:512413/1‑90 (MQ=255)                                                                           gcACACCGCCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGt  >  2:42758/1‑90 (MQ=255)                                                                           gcACACCGCCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGt  >  2:63668/1‑90 (MQ=255)                                                                           gcACACCGCCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGt  >  2:75552/1‑90 (MQ=255)                                                                                   |                                                                                  TTCTTCCCCTTTACGCACGCGCAGCACGTGGTAGTGCGGGGTTTCCATCTGATCGTTTGGCACAATTACACAGCGCACACCGTCCTGACGAGTTTCAATGGCATTTACCGCAGAACGTTTTTCATTCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGT  >  NZ_CP009273/1138342‑1138505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑