| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,148,053 | G→T | S220I (AGC→ATC) | fabF → | beta‑ketoacyl‑ACP synthase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,148,053 | 0 | G | T | 100.0% | 26.0 / NA | 8 | S220I (AGC→ATC) | fabF | beta‑ketoacyl‑ACP synthase II |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (3/5); total (3/5) | |||||||||||
AGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGT > NZ_CP009273/1147973‑1148086 | aGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGATCCGCCCGTg < 2:144507/90‑1 (MQ=255) gTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGATCCGCCCGTGGGATa > 2:84278/1‑90 (MQ=255) tggtggTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGATCCGCCCGTGGGATAAAGAGCGTGATGGttt < 2:361464/90‑1 (MQ=255) tggtTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGATCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGt < 1:84278/90‑1 (MQ=255) aCCTGCAATGATAACCCGCAAGCGGCGATCCGCCCg < 1:69132/36‑1 (MQ=37) aCCTGCAATGATAACCCGCAAGCGGCGATCCGCCCg > 2:69132/1‑36 (MQ=37) gCAATGATAACCCGCAAGCGGCGATCCGCCCGTGGGATAAAGAGCGTGAt < 1:242462/50‑1 (MQ=255) gCAATGATAACCCGCAAGCGGCGATCCGCCCGTGGGATAAAGAGCGTGAt > 2:242462/1‑50 (MQ=255) | AGCCAGTACGCCGCTGGGCGTTGGTGGTTTTGGCGCGGCACGTGCATTATCTACCCGCAATGATAACCCGCAAGCGGCGAGCCGCCCGTGGGATAAAGAGCGTGATGGTTTCGT > NZ_CP009273/1147973‑1148086 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |