Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,239,612	A→G	H124H (CAT→CAC)	ycgR ←	flagellar brake protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,239,612	0	A	G	100.0%	17.0 / NA	7	H124H (CAT→CAC)	ycgR	flagellar brake protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/5);  total (2/5)

GCCCATGCCGCCTAACGACAAATCATACAGGCGGAAACGTAACGTACTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTAC  >  NZ_CP009273/1239528‑1239677                                                                                     |                                                                  gcCCATGCCGCCTAACGACAAATCATACAGGCGGAAACGTAACGTACTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGGTGGAg                                                              <  2:144708/90‑1 (MQ=255)     aTGCCGCCTAACGACAAATCATACAGGCGGAAACGTAACGTACTGTTATCCGCCAGTTTGGGCTGGCAAAAATAAGGCGGGTgggggggg                                                          >  2:333472/1‑90 (MQ=255)          gccTAACGACAAATCATACAGGCGGAAACGTAACGTACTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGGTGGAGTGGGGCGga                                                     <  1:141977/90‑1 (MQ=255)                     aaTCATACAGGCGGAAACGTAACGTACTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGGGGGGGGGGGGGGGGGATGCGGgaaa                                          >  2:147998/1‑89 (MQ=255)                                             taCTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGGTGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATa                  <  1:430631/90‑1 (MQ=255)                                                            gTTTGGTCTGGCAAAAATAAGGCGGGTGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGcggta   <  2:81545/90‑1 (MQ=255)                                                             tttGGTCTGGCAAAAATAAGGCGGGTGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGcggtac  <  1:70672/90‑1 (MQ=255)                                                                                     |                                                                  GCCCATGCCGCCTAACGACAAATCATACAGGCGGAAACGTAACGTACTGTTATCCGCCAGTTTGGTCTGGCAAAAATAAGGCGGATGGAGTGGGGCGGAGATGCGGAAATATCGGCGTCGTTGTACAAACCATAAGGTGGGAGGCGGTAC  >  NZ_CP009273/1239528‑1239677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑